BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25294

Title: NMR structure of the protein NP_809137.1 from Bacteroides thetaiotaomicron

Deposition date: 2014-10-27 Original release date: 2014-11-10

Authors: Proudfoot, Andrew; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt

Citation: Proudfoot, Andrew; Wuthrich, Kurt; Serrano, Pedro; Geralt, Michael; Dutta, Samit. "NMR structure of the protein YP_002937094.1 from Eubacterium rectale"  Not known ., .-..

Assembly members:
entity, polymer, 118 residues, 13189.811 Da.

Natural source:   Common Name: CFB group bacteria   Taxonomy ID: 818   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacteroides thetaiotaomicron

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: GCELSDPDGLADPMKWSKVP SGLKNGELKVEAEGGSSLFA CKNYKSFWISSVKEEGKFKE NTSYKEFDGGWYLVKIEDNE LKVIINRNETNASRSFTVCV EAGNAFDEFKFVQDAAKQ

Data sets:
Data typeCount
1H chemical shifts762
13C chemical shifts384
15N chemical shifts124

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 118 residues - 13189.811 Da.

1   GLYCYSGLULEUSERASPPROASPGLYLEU
2   ALAASPPROMETLYSTRPSERLYSVALPRO
3   SERGLYLEULYSASNGLYGLULEULYSVAL
4   GLUALAGLUGLYGLYSERSERLEUPHEALA
5   CYSLYSASNTYRLYSSERPHETRPILESER
6   SERVALLYSGLUGLUGLYLYSPHELYSGLU
7   ASNTHRSERTYRLYSGLUPHEASPGLYGLY
8   TRPTYRLEUVALLYSILEGLUASPASNGLU
9   LEULYSVALILEILEASNARGASNGLUTHR
10   ASNALASERARGSERPHETHRVALCYSVAL
11   GLUALAGLYASNALAPHEASPGLUPHELYS
12   PHEVALGLNASPALAALALYSGLN

Samples:

sample_1: entity, [U-98% 13C; U-98% 15N], 1.2 mM; sodium chloride 50 mM; sodium phosphate 20 mM; sodium azide 5 mM; DTT, [U-99% 2H], 5 mM; H2O 95%; D2O 5%

sample_conditions_1: temperature: 298 K; pH: 6.0; pressure: 1 atm; ionic strength: 0.220 M

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
APSY 4D-HACANHsample_1isotropicsample_conditions_1
APSY 5D-CBCACONHsample_1isotropicsample_conditions_1
APSY 5D-HACACONHsample_1isotropicsample_conditions_1

Software:

CYANA, G ntert P., Luginbuhl, Guntert, Billeter and Wuthrich - refinement

TOPSPIN, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment

UNIO, Herrmann and Wuthrich - chemical shift assignment, structure solution

MDDNMR, Maxim Mayzel, Vladislav Orekhov - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AAO75331
REF NP_809137 WP_011107173

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts