BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25308

Title: Chemical shift assignments of human obscurin Ig58

Deposition date: 2014-11-03 Original release date: 2015-12-07

Authors: Wright, Nathan; Meyer, Logan

Citation: Wright, Nathan; Meyer, Logan; Kontrogianni-Konstantopoulos, Katia; Ackermann, Maegen; Hu, Rebecca. "Obscurin Ig58 disrupts myocyte development"  Not known ., .-..

Assembly members:
Ig58, polymer, 102 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Ig58: TAKNTVVRGLENVEALEGGE ALFECQLSQPEVAAHTWLLD DEPVRTSENAEVVFFENGLR HLLLLKNLRPQDSCRVTFLA GDMVTSAFLTVRGGLEHHHH HH

Data sets:
Data typeCount
13C chemical shifts376
15N chemical shifts91
1H chemical shifts625

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ig581

Entities:

Entity 1, Ig58 102 residues - Formula weight is not available

1   THRALALYSASNTHRVALVALARGGLYLEU
2   GLUASNVALGLUALALEUGLUGLYGLYGLU
3   ALALEUPHEGLUCYSGLNLEUSERGLNPRO
4   GLUVALALAALAHISTHRTRPLEULEUASP
5   ASPGLUPROVALARGTHRSERGLUASNALA
6   GLUVALVALPHEPHEGLUASNGLYLEUARG
7   HISLEULEULEULEULYSASNLEUARGPRO
8   GLNASPSERCYSARGVALTHRPHELEUALA
9   GLYASPMETVALTHRSERALAPHELEUTHR
10   VALARGGLYGLYLEUGLUHISHISHISHIS
11   HISHIS

Samples:

sample_1: Ig58, [U-99% 13C; U-99% 15N], 0.5 – 1.5 mM; Tris 20 mM; NaCl 50 mM; NaN3 0.3 mM; H20 90%; D20 10%

sample_conditions_1: ionic strength: 0.05 M; pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts