BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25334

Title: Hybrid structure of the Type 1 Pilus of Uropathogenic E.coli   PubMed: 26267365

Deposition date: 2014-11-13 Original release date: 2015-08-13

Authors: Habenstein, Birgit; Loquet, Antoine; Giller, Karin; Vasa, Suresh Kumar; Becker, Stefan; Habeck, Michael; Lange, Adam

Citation: Habenstein, Birgit; Loquet, Antoine; Vasa, Suresh Kumar; Giller, Karin; Becker, Stefan; Habeck, Michael; Lange, Adam. "Hybrid Structure of the Type 1 Pilus of Uropathogenic Escherichia coli"  Angew. Chem. Int. Ed. Engl. 54, 11691-11695 (2015).

Assembly members:
FimA, polymer, 160 residues, Formula weight is not available

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
FimA: MAATTVNGGTVHFKGEVVNA ACAVDAGSVDQTVQLGQVRT ASLAQEGATSSAVGFNIQLN DCDTNVASKAAVAFLGTAID AGHTNVLALQSSAAGSATNV GVQILDRTGAALTLDGATFS SETTLNNGTNTIPFQARYFA TGAATPGAANADATFKVQYQ

Data sets:
Data typeCount
13C chemical shifts639
15N chemical shifts161

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FimA1

Entities:

Entity 1, FimA 160 residues - Formula weight is not available

1   METALAALATHRTHRVALASNGLYGLYTHR
2   VALHISPHELYSGLYGLUVALVALASNALA
3   ALACYSALAVALASPALAGLYSERVALASP
4   GLNTHRVALGLNLEUGLYGLNVALARGTHR
5   ALASERLEUALAGLNGLUGLYALATHRSER
6   SERALAVALGLYPHEASNILEGLNLEUASN
7   ASPCYSASPTHRASNVALALASERLYSALA
8   ALAVALALAPHELEUGLYTHRALAILEASP
9   ALAGLYHISTHRASNVALLEUALALEUGLN
10   SERSERALAALAGLYSERALATHRASNVAL
11   GLYVALGLNILELEUASPARGTHRGLYALA
12   ALALEUTHRLEUASPGLYALATHRPHESER
13   SERGLUTHRTHRLEUASNASNGLYTHRASN
14   THRILEPROPHEGLNALAARGTYRPHEALA
15   THRGLYALAALATHRPROGLYALAALAASN
16   ALAASPALATHRPHELYSVALGLNTYRGLN

Samples:

sample_1: FimA, [U-100% 13C; U-100% 15N], mM; sodium phosphate 10 mM

sample_2: FimA, 2-glycerol 13C; U-100% 15N, uM; sodium phosphate 10 mM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 280 K

Experiments:

NameSampleSample stateSample conditions
NCACB 2Dsample_1solidsample_conditions_1
N(CO)CACB 3Dsample_1solidsample_conditions_1
NCACB 3Dsample_1solidsample_conditions_1
NCOCA 3Dsample_1solidsample_conditions_1
CANCO 3Dsample_1solidsample_conditions_1
NCACO 3Dsample_1solidsample_conditions_1
NCACO 2Dsample_1solidsample_conditions_1
N(CO)CACB 2Dsample_1solidsample_conditions_1
NCOCA 2Dsample_1solidsample_conditions_1
NCO 2Dsample_1solidsample_conditions_1
NCA 2Dsample_1solidsample_conditions_1
CC PDSDsample_1solidsample_conditions_1
CC PDSDsample_2solidsample_conditions_1
CC PDSDsample_2solidsample_conditions_1

Software:

CcpNMR_Analysis, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 850 MHz
  • Bruker Avance 600 MHz