BMRB Entry 25399
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25399
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Title: 1H, 15N, 13C resonance assignment of the aortic medial amyloid protein medin. PubMed: 26377205
Deposition date: 2014-12-17 Original release date: 2015-09-22
Authors: Davies, Hannah; Phelan, Marie; Madine, Jill
Citation: Davies, Hannah; Phelan, Marie; Madine, Jilllian. "1H, 15N and 13C assignment of the amyloidogenic protein medin using fast-pulsing NMR techniques" Biomol. NMR Assign. 10, 75-77 (2016).
Assembly members:
Medin, polymer, 50 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Medin: RLDKQGNFNAWVAGSYGNDQ
WLQVDLGSSKEVTGIITQGA
RNFGSVQFVA
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 144 |
15N chemical shifts | 48 |
1H chemical shifts | 66 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Medin | 1 |
Entities:
Entity 1, Medin 50 residues - Formula weight is not available
1 | ARG | LEU | ASP | LYS | GLN | GLY | ASN | PHE | ASN | ALA | |
2 | TRP | VAL | ALA | GLY | SER | TYR | GLY | ASN | ASP | GLN | |
3 | TRP | LEU | GLN | VAL | ASP | LEU | GLY | SER | SER | LYS | |
4 | GLU | VAL | THR | GLY | ILE | ILE | THR | GLN | GLY | ALA | |
5 | ARG | ASN | PHE | GLY | SER | VAL | GLN | PHE | VAL | ALA |
Samples:
sample_1: Medin, [U-98% 13C; U-98% 15N], 0.4 mM; sodium phosphate 20 mM; sodium chloride 20 mM; urea 8 M; TSP, [U-2H], 2 uM
sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aromatic TROSY | sample_1 | isotropic | sample_conditions_1 |
3D HNCO BEST | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB BEST | sample_1 | isotropic | sample_conditions_1 |
3D HCACO BEST | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CACB BEST | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY NUS | sample_1 | isotropic | sample_conditions_1 |
2D (HB)CB(CGCD)HD | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.1pl6, Bruker Biospin - collection, processing
Analysis v2.3.1, CCPN - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker Avance 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts