BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25409

Title: NMR resolved structure of VG16KRKP, an antimicrobial peptide in SDS

Deposition date: 2015-01-01 Original release date: 2015-02-02

Authors: Bhunia, Anirban; Datta, Aritreyee

Citation: Bhunia, Anirban; Datta, Aritreyee. "De-novo design of antimicrobial peptides:insights into membrane permeabilisation, lipopolysachharide fragmentation and application in plant disease control"  Not known ., .-..

Assembly members:
antimicrobial_peptide, polymer, 16 residues, 1765.171 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
antimicrobial_peptide: VARGWKRKCPLFGKGG

Data sets:
Data typeCount
1H chemical shifts93

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1antimicrobial_peptide1

Entities:

Entity 1, antimicrobial_peptide 16 residues - 1765.171 Da.

1   VALALAARGGLYTRPLYSARGLYSCYSPRO
2   LEUPHEGLYLYSGLYGLY

Samples:

sample_1: antimicrobial peptide 0.5 mM; H2O 90%; SDS 200 mM; D2O 10%

sample_conditions_1: temperature: 308 K; pH: 4.5; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

BMRB 25335 25408
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