BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25472

Title: Solution Structure of mu-SLPTX-Ssm6a

Deposition date: 2015-02-06 Original release date: 2016-02-15

Authors: Kim, Jaehyun; Kim, Jae Il

Citation: Kim, Jae hyun; Kim, Jae Il. "Solution Structure of mu-SLPTX-Ssm6a, a Gating Modifier of human Nav1.7 channels"  Biochemistry ., .-..

Assembly members:
entity, polymer, 46 residues, 5333.025 Da.

Natural source:   Common Name: centipede   Taxonomy ID: 55038   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Scolopendra subspinipes

Experimental source:   Production method: chemical synthesis   Host organism: scolopendra subspinipes mutilans

Entity Sequences (FASTA):
entity: ADNKCENSLRREIACGQCRD KVKTDGYFYECCTSDSTFKK CQDLLH

Data sets:
Data typeCount
1H chemical shifts233

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 46 residues - 5333.025 Da.

1   ALAASPASNLYSCYSGLUASNSERLEUARG
2   ARGGLUILEALACYSGLYGLNCYSARGASP
3   LYSVALLYSTHRASPGLYTYRPHETYRGLU
4   CYSCYSTHRSERASPSERTHRPHELYSLYS
5   CYSGLNASPLEULEUHIS

Samples:

sample_1: entity 1.0 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0 M; pH: 3.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz