BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25560

Title: Solution structure of the GBII-beta MRH domain W409A point mutant

Deposition date: 2015-04-01 Original release date: 2015-12-07

Authors: Olson, Linda; Peterson, Francis; Damhs, Nancy

Citation: Olson, Linda; Orsi, Ramiro; Peterson, Francis; Parodi, Armando; Kim, Jung-Ja; D'Alessio, Cecilia; Damhs, Nancy. "Crystal structure and functional analyses of glucosdidase II's lectin domain: Insgihts into oligomannose recognition"  Not known ., .-..

Assembly members:
entity, polymer, 94 residues, 10428.918 Da.

Natural source:   Common Name: fission yeast   Taxonomy ID: 4896   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Schizosaccharomyces pombe

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: YRAIKGMETKREIGGYTYKV VFYENVFQDSILLGNFASQE GNVLKYENGQSCANGPHRSA IVTVECGVENEIVSVLEAQK CEYLIKMKSPAACS

Data sets:
Data typeCount
13C chemical shifts356
15N chemical shifts96
1H chemical shifts619

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 94 residues - 10428.918 Da.

1   TYRARGALAILELYSGLYMETGLUTHRLYS
2   ARGGLUILEGLYGLYTYRTHRTYRLYSVAL
3   VALPHETYRGLUASNVALPHEGLNASPSER
4   ILELEULEUGLYASNPHEALASERGLNGLU
5   GLYASNVALLEULYSTYRGLUASNGLYGLN
6   SERCYSALAASNGLYPROHISARGSERALA
7   ILEVALTHRVALGLUCYSGLYVALGLUASN
8   GLUILEVALSERVALLEUGLUALAGLNLYS
9   CYSGLUTYRLEUILELYSMETLYSSERPRO
10   ALAALACYSSER

Samples:

sample_1: MRH GIIb, [U-99% 13C; U-99% 15N], 0.7 mM; imidazole, [U-99% 2H], 10 mM; sodium chloride 150 mM; sodium azide 0.02%; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.150 M; pH: 7.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

CYANA, Guentert P. - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts