BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25574

Title: Spatial Structure of Antimicrobial Peptide SmAMP2-2c from Seeds of Stellaria media

Deposition date: 2015-04-16 Original release date: 2016-06-27

Authors: Bozin, Timur; Bocharov, Eduard; Panova, Stanislava; Vassilevski, Alexander

Citation: Vassilevski, Alexander; Bozin, Timur; Musolyamov, Alexander; Panova, Stanislava; Slavokhotova, Anna; Mitkevich, Vladimir; Finkina, Ekaterina; Nikonorova, Alexandra; Ovchinnikova, Tatyana; Arseniev, Alexander; Babakov, Alexei; Bocharov, Eduard; Grishin, Eugene; Egorov, Tsezi. "Common chickweed (Stellaria media) antifungal peptides with chitin-binding domain provide unique plant defense strategy."  Molecular Plant ., .-..

Assembly members:
SmAMP2-2c, polymer, 30 residues, 3002.410 Da.

Natural source:   Common Name: Stellaria media   Taxonomy ID: 13274   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Stellaria media

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
SmAMP2-2c: AAGQCYRGRCSGGLCCSKYG YCGSGPAYCG

Data sets:
Data typeCount
13C chemical shifts81
1H chemical shifts162

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SmAMP2-2c1

Entities:

Entity 1, SmAMP2-2c 30 residues - 3002.410 Da.

1   ALAALAGLYGLNCYSTYRARGGLYARGCYS
2   SERGLYGLYLEUCYSCYSSERLYSTYRGLY
3   TYRCYSGLYSERGLYPROALATYRCYSGLY

Samples:

sample_inH2O: SmAMP2-2c 0.8 ± 0.1 mM; sodium acetate 20 ± 0.1 mM; sodium chloride 30 ± 0.1 mM

sample_inD2O: SmAMP2-2c 0.8 ± 0.1 mM; sodium acetate 20 ± 0.1 mM; sodium chloride 30 ± 0.1 mM

sample_4NOE: SmAMP2-2c 2 ± 0.1 mM; sodium acetate 10 ± 0.1 mM; sodium chloride 70 ± 0.1 mM

sample_conditions_30C: ionic strength: 0.08 M; pH: 4.5; pressure: 1 atm; temperature: 303 K

sample_conditions_10C: ionic strength: 0.05 M; pH: 4.5; pressure: 1 atm; temperature: 283 K

sample_conditions_40C: ionic strength: 0.05 M; pH: 4.5; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_inD2Oisotropicsample_conditions_30C
2D 1H-13C HSQCsample_inD2Oisotropicsample_conditions_30C
2D 1H-1H NOESYsample_4NOEisotropicsample_conditions_30C
2D 1H-1H TOCSYsample_4NOEisotropicsample_conditions_40C
2D 1H-1H TOCSYsample_inH2Oisotropicsample_conditions_30C
2D 1H-1H TOCSYsample_inH2Oisotropicsample_conditions_10C

Software:

Molmol, Koradi, Billeter and Wuthrich - molecule viewer

CYANA v2.1, Guntert, Braun and Wuthrich - structure solution

ACME, Delaglio, Zhengrong and Bax - J-coupling measurement

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 700 MHz
  • Bruker DRX 800 MHz

Related Database Links:

GB CBJ21249