BMRB Entry 26883
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26883
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Title: HIV-1 Capsid-SP1 in immature virus-like particles PubMed: 27593947
Deposition date: 2016-08-29 Original release date: 2017-02-16
Authors: Ganser-Pornillos, Barbie; Zadrozny, Kaneil; Yeager, Mark
Citation: Bayro, Marvin; Ganser-Pornillos, Barbie; Zadrozny, Kaneil; Yeager, Mark; Tycko, Robert. "Helical Conformation in the CA-SP1 Junction of the Immature HIV-1 Lattice Determined from Solid-State NMR of Virus-like Particles" J. Am. Chem. Soc. 138, 12029-12032 (2016).
Assembly members:
dMA-CA-SP1-NC, polymer, 348 residues, Formula weight is not available
Natural source: Common Name: viruses Taxonomy ID: 12721 Superkingdom: Viruses Kingdom: not available Genus/species: Lentivirus not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
dMA-CA-SP1-NC: MGARASVLSGGELDKALDKI
EEEQNKSKKKAQQAAADTGN
NSQVSQNYPIVQNLQGQMVH
QAISPRTLNAWVKVVEEKAF
SPEVIPMFSALSEGATPQDL
NTMLNTVGGHQAAMQMLKET
INEEAAEWDRLHPVHAGPIA
PGQMREPRGSDIAGTTSTLQ
EQIGWMTHNPPIPVGEIYKR
WIILGLNKIVRMYSPTSILD
IRQGPKEPFRDYVDRFYKTL
RAEQASQEVKNWMTETLLVQ
NANPDCKTILKALGPGATLE
EMMTACQGVGGPGHKARVLA
EAMSQVCNPATIMIQKGNFR
NQRKTVKCFNCGKEGHIAKN
CRAPRKKGCWKCGKEGHQMK
DCTERQAN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 298 |
| 15N chemical shifts | 121 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | virus-like particles | 1 |
Entities:
Entity 1, virus-like particles 348 residues - Formula weight is not available
| 1 | MET | GLY | ALA | ARG | ALA | SER | VAL | LEU | SER | GLY | ||||
| 2 | GLY | GLU | LEU | ASP | LYS | ALA | LEU | ASP | LYS | ILE | ||||
| 3 | GLU | GLU | GLU | GLN | ASN | LYS | SER | LYS | LYS | LYS | ||||
| 4 | ALA | GLN | GLN | ALA | ALA | ALA | ASP | THR | GLY | ASN | ||||
| 5 | ASN | SER | GLN | VAL | SER | GLN | ASN | TYR | PRO | ILE | ||||
| 6 | VAL | GLN | ASN | LEU | GLN | GLY | GLN | MET | VAL | HIS | ||||
| 7 | GLN | ALA | ILE | SER | PRO | ARG | THR | LEU | ASN | ALA | ||||
| 8 | TRP | VAL | LYS | VAL | VAL | GLU | GLU | LYS | ALA | PHE | ||||
| 9 | SER | PRO | GLU | VAL | ILE | PRO | MET | PHE | SER | ALA | ||||
| 10 | LEU | SER | GLU | GLY | ALA | THR | PRO | GLN | ASP | LEU | ||||
| 11 | ASN | THR | MET | LEU | ASN | THR | VAL | GLY | GLY | HIS | ||||
| 12 | GLN | ALA | ALA | MET | GLN | MET | LEU | LYS | GLU | THR | ||||
| 13 | ILE | ASN | GLU | GLU | ALA | ALA | GLU | TRP | ASP | ARG | ||||
| 14 | LEU | HIS | PRO | VAL | HIS | ALA | GLY | PRO | ILE | ALA | ||||
| 15 | PRO | GLY | GLN | MET | ARG | GLU | PRO | ARG | GLY | SER | ||||
| 16 | ASP | ILE | ALA | GLY | THR | THR | SER | THR | LEU | GLN | ||||
| 17 | GLU | GLN | ILE | GLY | TRP | MET | THR | HIS | ASN | PRO | ||||
| 18 | PRO | ILE | PRO | VAL | GLY | GLU | ILE | TYR | LYS | ARG | ||||
| 19 | TRP | ILE | ILE | LEU | GLY | LEU | ASN | LYS | ILE | VAL | ||||
| 20 | ARG | MET | TYR | SER | PRO | THR | SER | ILE | LEU | ASP | ||||
| 21 | ILE | ARG | GLN | GLY | PRO | LYS | GLU | PRO | PHE | ARG | ||||
| 22 | ASP | TYR | VAL | ASP | ARG | PHE | TYR | LYS | THR | LEU | ||||
| 23 | ARG | ALA | GLU | GLN | ALA | SER | GLN | GLU | VAL | LYS | ||||
| 24 | ASN | TRP | MET | THR | GLU | THR | LEU | LEU | VAL | GLN | ||||
| 25 | ASN | ALA | ASN | PRO | ASP | CYS | LYS | THR | ILE | LEU | ||||
| 26 | LYS | ALA | LEU | GLY | PRO | GLY | ALA | THR | LEU | GLU | ||||
| 27 | GLU | MET | MET | THR | ALA | CYS | GLN | GLY | VAL | GLY | ||||
| 28 | GLY | PRO | GLY | HIS | LYS | ALA | ARG | VAL | LEU | ALA | ||||
| 29 | GLU | ALA | MET | SER | GLN | VAL | CYS | ASN | PRO | ALA | ||||
| 30 | THR | ILE | MET | ILE | GLN | LYS | GLY | ASN | PHE | ARG | ||||
| 31 | ASN | GLN | ARG | LYS | THR | VAL | LYS | CYS | PHE | ASN | ||||
| 32 | CYS | GLY | LYS | GLU | GLY | HIS | ILE | ALA | LYS | ASN | ||||
| 33 | CYS | ARG | ALA | PRO | ARG | LYS | LYS | GLY | CYS | TRP | ||||
| 34 | LYS | CYS | GLY | LYS | GLU | GLY | HIS | GLN | MET | LYS | ||||
| 35 | ASP | CYS | THR | GLU | ARG | GLN | ALA | ASN |
Samples:
sample_1: dMA-CA-SP1-NC, [U-98% 13C; U-98% 15N], 13 mM
sample_conditions_1: ionic strength: 1 M; pH: 6.5; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D NCACX | sample_1 | isotropic | sample_conditions_1 |
| 3D NCOCX | sample_1 | isotropic | sample_conditions_1 |
| 3D CONCA | sample_1 | isotropic | sample_conditions_1 |
| 2D CC | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian Infinity 600 MHz