BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27506

Title: Chemical shift assignment of the viral protein genome-linked (VPg) from Potato virus Y   PubMed: 30242622

Deposition date: 2018-06-05 Original release date: 2018-10-03

Authors: Coutinho de Oliveira, Luciana; Volpon, Laurent; Osborne, Michael; Borden, Katherine

Citation: Coutinho de Oliveira, Luciana; Volpon, Laurent; Osborne, Michael; Borden, Katherine. "Chemical shift assignment of the viral protein genome-linked (VPg) from potato virus Y"  Biomol. NMR Assignments 13, 9-13 (2019).

Assembly members:
VPg_from_potato_virus_Y, polymer, 153 residues, Formula weight is not available

Natural source:   Common Name: potato virus Y   Taxonomy ID: 12216   Superkingdom: Viruses   Kingdom: not available   Genus/species: Potyvirus not available

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
VPg_from_potato_virus_Y: GSSAYRKKGKGKGTTVGMGK SSRRFINMYGFDPTEYSFIQ FVDPLTGAQIEENVYADIRD IQERFSEVRKKMVENDDIEM QALGSNTTIHAYFRKDWSDK ALKIDLMPHNPLKVCDKTNG IAKFPERELELRQTGPAVEV DVKDIPAQEVEHE

Data sets:
Data typeCount
13C chemical shifts481
15N chemical shifts123
1H chemical shifts703

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1VPg(-37)1

Entities:

Entity 1, VPg(-37) 153 residues - Formula weight is not available

The first 2 residues (GS) come from the tag.

1   GLYSERSERALATYRARGLYSLYSGLYLYS
2   GLYLYSGLYTHRTHRVALGLYMETGLYLYS
3   SERSERARGARGPHEILEASNMETTYRGLY
4   PHEASPPROTHRGLUTYRSERPHEILEGLN
5   PHEVALASPPROLEUTHRGLYALAGLNILE
6   GLUGLUASNVALTYRALAASPILEARGASP
7   ILEGLNGLUARGPHESERGLUVALARGLYS
8   LYSMETVALGLUASNASPASPILEGLUMET
9   GLNALALEUGLYSERASNTHRTHRILEHIS
10   ALATYRPHEARGLYSASPTRPSERASPLYS
11   ALALEULYSILEASPLEUMETPROHISASN
12   PROLEULYSVALCYSASPLYSTHRASNGLY
13   ILEALALYSPHEPROGLUARGGLULEUGLU
14   LEUARGGLNTHRGLYPROALAVALGLUVAL
15   ASPVALLYSASPILEPROALAGLNGLUVAL
16   GLUHISGLU

Samples:

VPg(-37)-15N13C-H2O: VPg from potato virus Y, [U-100% 13C; U-100% 15N], 400 uM; sodium phosphate 50 mM; sodium chloride 150 mM; DTT 1 mM; sodium azide 0.02 % v/v

VPg(-37)-2H15N13C-H2O: VPg from potato virus Y, [U-13C; U-15N; U-2H], 400 uM; sodium phosphate 50 mM; sodium chloride 150 mM; DTT 1 mM; sodium azide 0.02 % v/v

VPg(-37)-2H15N13C-D2O: VPg from potato virus Y, [U-13C; U-15N; U-2H], 400 uM; sodium phosphate 50 mM; sodium chloride 150 mM; DTT 1 mM; sodium azide 0.02 % v/v

VPg(-37)-15N13C-D2O: VPg from potato virus Y, [U-100% 13C; U-100% 15N], 400 uM; sodium phosphate 50 mM; sodium chloride 150 mM; DTT 1 mM; sodium azide 0.02 % v/v

sample_condition: pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCVPg(-37)-15N13C-H2Oisotropicsample_condition
2D 1H-15N HSQCVPg(-37)-2H15N13C-H2Oisotropicsample_condition
2D 1H-15N HSQCVPg(-37)-2H15N13C-H2Oisotropicsample_condition
2D 1H-13C HSQC aliphaticVPg(-37)-15N13C-D2Oisotropicsample_condition
2D 1H-13C HSQC aromaticVPg(-37)-15N13C-D2Oisotropicsample_condition
3D HNCAVPg(-37)-15N13C-H2Oisotropicsample_condition
3D HN(CO)CAVPg(-37)-15N13C-H2Oisotropicsample_condition
3D HNCACBVPg(-37)-15N13C-H2Oisotropicsample_condition
3D CBCA(CO)NHVPg(-37)-15N13C-H2Oisotropicsample_condition
3D HNCOVPg(-37)-15N13C-H2Oisotropicsample_condition
3D HN(CA)COVPg(-37)-15N13C-H2Oisotropicsample_condition
3D HNCACBVPg(-37)-2H15N13C-H2Oisotropicsample_condition
3D CBCA(CO)NHVPg(-37)-2H15N13C-H2Oisotropicsample_condition
3D HNCOVPg(-37)-2H15N13C-H2Oisotropicsample_condition
3D HN(CA)COVPg(-37)-2H15N13C-H2Oisotropicsample_condition
3D HCCH-TOCSYVPg(-37)-2H15N13C-D2Oisotropicsample_condition
3D H(CCO)NHVPg(-37)-15N13C-H2Oisotropicsample_condition
3D C(CO)NHVPg(-37)-2H15N13C-H2Oisotropicsample_condition
3D 1H-15N NOESYVPg(-37)-15N13C-H2Oisotropicsample_condition
3D 1H-13C NOESY aromaticVPg(-37)-2H15N13C-D2Oisotropicsample_condition
3D (H)N(COCA)NHVPg(-37)-2H15N13C-H2Oisotropicsample_condition

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts