BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30170

Title: TIRAP phosphoinositide-binding motif   PubMed: 28225045

Deposition date: 2016-09-05 Original release date: 2017-03-02

Authors: Capelluto, D.; Ellena, J.; Armstrong, G.; Zhao, X.; Xiao, S.

Citation: Zhao, X.; Xiong, W.; Xiao, S.; Ellena, J.; Armstrong, G.; Finkielstein, C.; Capelluto, D.. "Membrane targeting of TIRAP is negatively regulated by phosphorylation in its phosphoinositide-binding motif"  Sci. Rep. 7, 43043-43043 (2017).

Assembly members:
entity_1, polymer, 21 residues, 2550.200 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Entity Sequences (FASTA):
entity_1: KKPLGKMADWFRQTLLKKPK K

Data sets:
Data typeCount
13C chemical shifts61
15N chemical shifts19
1H chemical shifts19

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 21 residues - 2550.200 Da.

1   LYSLYSPROLEUGLYLYSMETALAASPTRP
2   PHEARGGLNTHRLEULEULYSLYSPROLYS
3   LYS

Samples:

sample_1: TIRAP PBM, [U-99% 13C; U-99% 15N], 0.4 ± 0.04 mM

sample_conditions_1: ionic strength: 60 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D TOCSYHSQCsample_1isotropicsample_conditions_1
3D NOESYHSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CS-Rosetta v3.5, Shen, Vernon, Baker and Bax - refinement, structure calculation

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AvanceIII 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts