BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 30193

Title: Solution Structure of the N-terminal DNA-binding domain of the master biofilm-regulator SinR from Bacillus subtilis

Deposition date: 2016-10-13 Original release date: 2017-10-19

Authors: Draughn, G.; Bobay, B.; Stowe, S.; Thompson, R.; Cavanagh, J.

Citation: Draughn, G.; Bobay, B.; Stowe, S.; Thompson, R.; Cavanagh, J.. "Solution Structure of the N-terminal DNA-binding domain of the master biofilm-regulator SinR from Bacillus subtilis"  . ., .-..

Assembly members:
HTH-type transcriptional regulator SinR, polymer, 69 residues, 7847.908 Da.

Natural source:   Common Name: firmicutes   Taxonomy ID: 224308   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
HTH-type transcriptional regulator SinR: MIGQRIKQYRKEKGYSLSEL AEKAGVAKSYLSSIERNLQT NPSIQFLEKVSAVLDVSVHT LLDEKHETE

Data sets:
Data typeCount
13C chemical shifts309
15N chemical shifts66
1H chemical shifts465

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 69 residues - 7847.908 Da.

1   METILEGLYGLNARGILELYSGLNTYRARG
2   LYSGLULYSGLYTYRSERLEUSERGLULEU
3   ALAGLULYSALAGLYVALALALYSSERTYR
4   LEUSERSERILEGLUARGASNLEUGLNTHR
5   ASNPROSERILEGLNPHELEUGLULYSVAL
6   SERALAVALLEUASPVALSERVALHISTHR
7   LEULEUASPGLULYSHISGLUTHRGLU

Samples:

sample_1: MES 20 mM; SinrN, [U-15N], 1 mM; sodium azide 0.02%; sodium chloride 200 mM; H2O 90%; D2O 10%

sample_2: MES 20 mM; SinrN, [U-13C; U-15N], 1 mM; sodium azide 0.02%; sodium chloride 200 mM; H2O 90%; D2O 10%

sample_3: MES 20 mM; SinRN, [U-13C; U-15N], 1 mM; sodium azide 0.02%; sodium chloride 200 mM; D2O 100%

sample_conditions_1: ionic strength: 200 mM; pH: 6.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_3isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1

Software:

AMBER v14, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, and Kollman - refinement

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 700 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts