BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30441

Title: TFE-induced NMR structure of a novel bioactive peptide (PaDBS1R7) derived from a Pyrobaculum aerophilum ribosomal protein (L39e)

Deposition date: 2018-03-21 Original release date: 2019-09-17

Authors: Cardoso, M.; Chan, L.; Candido, E.; Craik, D.; Franco, O.

Citation: Cardoso, M.; Chan, L.; Candido, E.; Oshiro, K.; Torres, M.; de la Fuente-Nunez, C.; Felicio, M.; Ribeiro, S.; Porto, W.; Santos, N.; Lu, T.; Craik, D.; Franco, O.. "Decoding the function and structure of three peptides derived from a Pyrobaculum aerophilum ribosomal protein"  . ., .-..

Assembly members:
entity_1, polymer, 19 residues, 2272.948 Da.

Natural source:   Common Name: Pyrobaculum aerophilum   Taxonomy ID: 178306   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrobaculum aerophilum

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: PMARNKPKILKRILAKIFK

Data sets:
Data typeCount
13C chemical shifts69
15N chemical shifts17
1H chemical shifts154

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 19 residues - 2272.948 Da.

1   PROMETALAARGASNLYSPROLYSILELEU
2   LYSARGILELEUALALYSILEPHELYS

Samples:

sample_1: PaDBS1R7 1 mM; TFE 30%; H2O 60%; D2O 10%; DSS 10%

sample_conditions_1: pH: 4.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1anisotropicsample_conditions_1
2D 1H-1H NOESYsample_1anisotropicsample_conditions_1
2D 1H-15N HSQCsample_1anisotropicsample_conditions_1
2D 1H-13C HSQCsample_1anisotropicsample_conditions_1

Software:

CcpNMR, CCPN - chemical shift assignment, peak picking

TALOS, Cornilescu, Delaglio and Bax - geometry optimization

CYANA, Guntert, Mumenthaler and Wuthrich - data analysis

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation

NMR spectrometers:

  • Bruker Avance 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts