BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 30504

Title: Solution NMR Structure of a Class I Hydrophobin from Phanerochaete carnosa

Deposition date: 2018-07-31 Original release date: 2019-08-02

Authors: Kenward, C.; Langelaan, D.

Citation: Kenward, C.; Langelaan, D.. "Solution NMR Structure of a Class I Hydrophobin from Phanerochaete carnosa"  . ., .-..

Assembly members:
entity_1, polymer, 84 residues, 8286.277 Da.

Natural source:   Common Name: White-rot fungus   Taxonomy ID: 650164   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Phanerochaete carnosa

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli DH1

Entity Sequences (FASTA):
entity_1: GSWGQCSTGSIQCCQNVVPG DSDLGTLLLDELGIVLEDPT VLIGDGCDPITVAGSSDACS ATAVCCSDNNVSGVIAIGCL PVTL

Data sets:
Data typeCount
13C chemical shifts326
15N chemical shifts85
1H chemical shifts519

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 84 residues - 8286.277 Da.

1   GLYSERTRPGLYGLNCYSSERTHRGLYSER
2   ILEGLNCYSCYSGLNASNVALVALPROGLY
3   ASPSERASPLEUGLYTHRLEULEULEUASP
4   GLULEUGLYILEVALLEUGLUASPPROTHR
5   VALLEUILEGLYASPGLYCYSASPPROILE
6   THRVALALAGLYSERSERASPALACYSSER
7   ALATHRALAVALCYSCYSSERASPASNASN
8   VALSERGLYVALILEALAILEGLYCYSLEU
9   PROVALTHRLEU

Samples:

sample_1: PC1, [U-13C; U-15N], 800 uM; MES 20 mM; sodium chloride 50 mM; sodium azide 1 mM

sample_2: PC1, [U-15N], 110 uM; MES 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 303.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

ARIA v2.3.1, Linge, O'Donoghue and Nilges - refinement, structure calculation

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Analysis, CCPN - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker AvanceIII 800 MHz
  • Bruker AvanceIII 700 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts