BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30718

Title: Solution NMR of Prochlorosin 2.8 produced by Prochlorococcus MIT 9313

Deposition date: 2020-01-24 Original release date: 2020-07-05

Authors: Bobeica, S.; Acedo, J.; van der Donk, W.; Zhu, L.

Citation: Bobeica, S.; Acedo, J.; van der Donk, W.; Zhu, L.. "Solution NMR of Prochlorosin 2.8 produced by Prochlorococcus MIT 9313"  to be published ., .-..

Assembly members:
entity_1, polymer, 19 residues, 2057.289 Da.

Natural source:   Common Name: Prochlorococcus marinus   Taxonomy ID: 74547   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Prochlorococcus marinus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: AACHNHAPXMPPXYWEGEC

Data sets:
Data typeCount
13C chemical shifts41
15N chemical shifts15
1H chemical shifts103

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 19 residues - 2057.289 Da.

1   ALAALACYSHISASNHISALAPRODALMET
2   PROPRODALTYRTRPGLUGLYGLUCYS

Samples:

sample_1: Prochlorosin 2.8 33 ± 0.3 mM

sample_2: Prochlorosin 2.8 33 ± 0.3 mM

sample_3: Prochlorosin 2.8 33 ± 0.3 mM

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

sample_conditions_2: pH: 6.0; pressure: 1 atm; temperature: 298 K

sample_conditions_3: pH: 6.0; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_2
2D 1H-1H NOESYsample_2isotropicsample_conditions_2
2D 1H-13C HSQCsample_2isotropicsample_conditions_2
2D 1H-15N HSQCsample_2isotropicsample_conditions_2
2D 1H-1H TOCSYsample_3isotropicsample_conditions_3
2D 1H-1H NOESYsample_3isotropicsample_conditions_3

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Sparky, Goddard - chemical shift assignment, peak picking

X-PLOR NIH v2.51, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

NMR spectrometers:

  • Agilent VNMRS 750 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts