BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 34106

Title: NMR solution structure of ubl5 domain from polyubiquitin locus of T.thermophila.   PubMed: 30690122

Deposition date: 2017-02-27 Original release date: 2018-03-16

Authors: Chiarini, V.; Tossavainen, H.

Citation: Chiarini, Valerio; Tossavainen, Helena; Sharma, Vivek; Colotti, Gianni. "NMR structure of a non-conjugatable, ADP-ribosylation associated, ubiquitin-like domain from Tetrahymena thermophila polyubiquitin locus"  Biochim. Biophys. Acta Gen. Subj. 1863, 749-759 (2019).

Assembly members:
entity_1, polymer, 81 residues, 9457.851 Da.

Natural source:   Common Name: Tetrahymena thermophila   Taxonomy ID: 312017   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Tetrahymena thermophila

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: SHMQVSIEFQNGEKLKFNVQ PSHKILDIKEMIFKKTNINV KDQSLKFAGNEMINQKTLSD YSIIDSTEEFTLHLETKLDL M

Data sets:
Data typeCount
13C chemical shifts391
15N chemical shifts89
1H chemical shifts617

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 81 residues - 9457.851 Da.

1   SERHISMETGLNVALSERILEGLUPHEGLN
2   ASNGLYGLULYSLEULYSPHEASNVALGLN
3   PROSERHISLYSILELEUASPILELYSGLU
4   METILEPHELYSLYSTHRASNILEASNVAL
5   LYSASPGLNSERLEULYSPHEALAGLYASN
6   GLUMETILEASNGLNLYSTHRLEUSERASP
7   TYRSERILEILEASPSERTHRGLUGLUPHE
8   THRLEUHISLEUGLUTHRLYSLEUASPLEU
9   MET

Samples:

sample_1: T.thermophila BUBL1-ubl5, [U-13C; U-15N], 4 mM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 0.06 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HCC(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CcpNMR, CCPN - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

AMBER, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

  • Bruker AvanceIII 850 MHz
  • Bruker AvanceIII 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts