BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 34220

Title: NMR Solution Structure of MINA-1(254-334)   PubMed: 30728462

Deposition date: 2017-12-19 Original release date: 2019-01-28

Authors: Michel, E.; Allain, F.

Citation: Sendoel, Ataman; Subasic, Deni; Ducoli, Luca; Keller, Martin; Michel, Erich; Kohler, Ines; Singh, Kapil Dev; Zheng, Xue; Brummer, Anneke; Imig, Jochen; Kishore, Shivendra; Wu, Yibo; Kanitz, Alexander; Kaech, Andres; Mittal, Nitish; Matia-Gonzalez, Ana; Gerber, Andre; Zavolan, Mihaela; Aebersold, Ruedi; Hall, Jonathan; Allain, Frederic H-T; Hengartner, Michael. "MINA-1 and WAGO-4 are part of regulatory network coordinating germ cell death and RNAi in C. elegans"  Cell Death Differ. ., .-. (2019).

Assembly members:
entity_1, polymer, 86 residues, 9634.081 Da.

Natural source:   Common Name: C. elegans   Taxonomy ID: 6239   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Caenorhabditis elegans

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):
entity_1: SGSHMKTCVVEKIKQWIPTT EVGKILGNRAAVKKHIERQF NCVITVHTEVQSSFGATPVE IVAQNKEQCQEARNAVMSLM QSHQDK

Data sets:
Data typeCount
13C chemical shifts286
15N chemical shifts98
1H chemical shifts608

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 86 residues - 9634.081 Da.

1   SERGLYSERHISMETLYSTHRCYSVALVAL
2   GLULYSILELYSGLNTRPILEPROTHRTHR
3   GLUVALGLYLYSILELEUGLYASNARGALA
4   ALAVALLYSLYSHISILEGLUARGGLNPHE
5   ASNCYSVALILETHRVALHISTHRGLUVAL
6   GLNSERSERPHEGLYALATHRPROVALGLU
7   ILEVALALAGLNASNLYSGLUGLNCYSGLN
8   GLUALAARGASNALAVALMETSERLEUMET
9   GLNSERHISGLNASPLYS

Samples:

sample_1: MINA-1(254-334), [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DTT 2 mM; EDTA 0.5 mM

sample_conditions_1: ionic strength: 50 mM; pH: 5.0; pressure: 1 atm; temperature: 293.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

ATNOS, Herrmann, Guntert and Wuthrich - peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

AMBER, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 500 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts