BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 36111

Title: Solution structure of yeast Fra2   PubMed: 29524511

Deposition date: 2017-08-03 Original release date: 2018-03-26

Authors: Tang, Y.; Chi, C.; Zhang, J.; Dai, Y.; Abdalla, M.; Chen, Y.; Zhou, C.

Citation: Chi, C.; Tang, Y.; Zhang, J.; Dai, Y.; Abdalla, M.; Chen, Y.; Zhou, C.. "Structural and Biochemical Insights into the Multiple Functions of Yeast Grx3"  J. Mol. Biol. 2836, 30110-30114 (2018).

Assembly members:
BolA-like protein 2, polymer, 93 residues, 10810.320 Da.

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 559292   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
BolA-like protein 2: MPVTEQGLRERIESAIPQVY HIIVTDLSYGCGQSFDIVVV SDFFQGKSKLMRSRAVNKAV KEELQEIHAFSCKCYTEEEW SKIVVLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts254
15N chemical shifts76
1H chemical shifts539

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 93 residues - 10810.320 Da.

1   METPROVALTHRGLUGLNGLYLEUARGGLU
2   ARGILEGLUSERALAILEPROGLNVALTYR
3   HISILEILEVALTHRASPLEUSERTYRGLY
4   CYSGLYGLNSERPHEASPILEVALVALVAL
5   SERASPPHEPHEGLNGLYLYSSERLYSLEU
6   METARGSERARGALAVALASNLYSALAVAL
7   LYSGLUGLULEUGLNGLUILEHISALAPHE
8   SERCYSLYSCYSTYRTHRGLUGLUGLUTRP
9   SERLYSILEVALVALLEUGLUHISHISHIS
10   HISHISHIS

Samples:

sample_1: Fra2, [U-13C; U-15N], 0.8 mM; NaCl 150 mM; H2O 90%; D2O, [U-2H], 10%

sample_2: Fra2, [U-13C; U-15N], 0.8 mM; NaCl 150 mM; D2O, [U-2H], 100%

sample_conditions_1: ionic strength: 150 mM; pH: 8.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 15N-1H HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D (H)C(CO)NH-TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CBCA)-(CO)NHsample_1isotropicsample_conditions_1
3D H(C)(CO)NH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

ProcheckNMR, Laskowski and MacArthur - refinement

SPARKY, Goddard - chemical shift assignment, peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure calculation

NMR spectrometers:

  • Bruker DMX 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts