BMRB Entry 4229
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4229
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Artificial Fe8S8 ferredoxin: the D13C variant of Bacillus schlegelii Fe7S8 ferredoxin
Deposition date: 1998-08-20 Original release date: 2000-05-01
Authors: Aono, S.; Bentrop, D.; Bertini, I.; Cosenza, G.; Luchinat, C.
Citation: Aono, S.; Bentrop, D.; Bertini, I.; Cosenza, G.; Luchinat, C.. "Solution structure of an artficial Fe8S8 ferredoxin: the D13C variant of Bacillus schlegelii Fe7S8 ferredoxin"" Eur. J. Biochem. 258, 502-514 (1998).
Assembly members:
ferredoxin, polymer, 77 residues, Formula weight is not available
IRON/SULFUR CLUSTER, non-polymer, 351.640 Da.
Natural source: Common Name: not available Taxonomy ID: 1484 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus schlegelii
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ferredoxin: AYVITEPCIGTKCASCVEVC
PVDCIHEGEDQYYIDPDVCI
DCGACEAVCPVSAIYHEDFV
PEEWKSYIQKNRDFFKK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 586 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Fe8S8 Fd | 1 |
| 2 | FeS4, cluster 1 | 2 |
| 3 | FeS4, cluster 2 | 2 |
Entities:
Entity 1, Fe8S8 Fd 77 residues - Formula weight is not available
| 1 | ALA | TYR | VAL | ILE | THR | GLU | PRO | CYS | ILE | GLY | ||||
| 2 | THR | LYS | CYS | ALA | SER | CYS | VAL | GLU | VAL | CYS | ||||
| 3 | PRO | VAL | ASP | CYS | ILE | HIS | GLU | GLY | GLU | ASP | ||||
| 4 | GLN | TYR | TYR | ILE | ASP | PRO | ASP | VAL | CYS | ILE | ||||
| 5 | ASP | CYS | GLY | ALA | CYS | GLU | ALA | VAL | CYS | PRO | ||||
| 6 | VAL | SER | ALA | ILE | TYR | HIS | GLU | ASP | PHE | VAL | ||||
| 7 | PRO | GLU | GLU | TRP | LYS | SER | TYR | ILE | GLN | LYS | ||||
| 8 | ASN | ARG | ASP | PHE | PHE | LYS | LYS |
Entity 2, FeS4, cluster 1 - Fe4 S4 - 351.640 Da.
| 1 | SF4 |
Samples:
sample_1: ferredoxin2 – 3.3 mM
sample_cond_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 300 K
sample_cond_2: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 282 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | sample_1 | not available | not available |
| DQF-COSY | sample_1 | not available | not available |
| TOCSY | sample_1 | not available | not available |
| 1D-NOE | sample_1 | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz