BMRB Entry 4242
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4242
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Title: Motile Major Sperm Protein (MSP) of Ascaris suum
Deposition date: 1998-10-09 Original release date: 1999-01-25
Authors: Haaf, Andreas; LeClaire, Lawrence; Roberts, Gregory; Kent, Helen; Roberts, Thomas; Stewart, Murray; Neuhaus, David
Citation: Haaf, Andreas; LeClaire, Lawrence; Roberts, Gregory; Kent, Helen; Roberts, Thomas; Stewart, Murray; Neuhaus, David. "Solution Structure of the Motile Major Sperm Protein (MSP) of Ascaris suum - Evidence for Two Manganese Binding Sites and the Possible Role of Divalent Cations in Filament Formation." J. Mol. Biol. 284, 1611-1623 (1998).
Assembly members:
Major Sperm Protein, polymer, 126 residues, Formula weight is not available
Natural source: Common Name: Pig Roundworm Taxonomy ID: 6253 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Ascaris suum
Experimental source: Production method: recombinant_technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Major Sperm Protein: AQSVPPGDINTQPSQKIVFN
APYDDKHTYHIKITNAGGRR
IGWAIKTTNMRRLSVDPPCG
VLDPKEKVLMAVSCDTFNAA
TEDLNNDRITIEWTNTPDGA
AKQFRREWFQGDGMVRRKNL
PIEYNL
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 410 |
15N chemical shifts | 132 |
1H chemical shifts | 825 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | MSP subunit A | 1 |
2 | MSP subunit B | 1 |
Entities:
Entity 1, MSP subunit A 126 residues - Formula weight is not available
1 | ALA | GLN | SER | VAL | PRO | PRO | GLY | ASP | ILE | ASN | ||||
2 | THR | GLN | PRO | SER | GLN | LYS | ILE | VAL | PHE | ASN | ||||
3 | ALA | PRO | TYR | ASP | ASP | LYS | HIS | THR | TYR | HIS | ||||
4 | ILE | LYS | ILE | THR | ASN | ALA | GLY | GLY | ARG | ARG | ||||
5 | ILE | GLY | TRP | ALA | ILE | LYS | THR | THR | ASN | MET | ||||
6 | ARG | ARG | LEU | SER | VAL | ASP | PRO | PRO | CYS | GLY | ||||
7 | VAL | LEU | ASP | PRO | LYS | GLU | LYS | VAL | LEU | MET | ||||
8 | ALA | VAL | SER | CYS | ASP | THR | PHE | ASN | ALA | ALA | ||||
9 | THR | GLU | ASP | LEU | ASN | ASN | ASP | ARG | ILE | THR | ||||
10 | ILE | GLU | TRP | THR | ASN | THR | PRO | ASP | GLY | ALA | ||||
11 | ALA | LYS | GLN | PHE | ARG | ARG | GLU | TRP | PHE | GLN | ||||
12 | GLY | ASP | GLY | MET | VAL | ARG | ARG | LYS | ASN | LEU | ||||
13 | PRO | ILE | GLU | TYR | ASN | LEU |
Samples:
sample_one: Major Sperm Protein2.5 3.5 mM; D2O 5%; H2O 95%
sample_two: Major Sperm Protein, [U-98%15N], 2.5 3.5 mM; D2O 5%; H2O 95%
sample_three: Major Sperm Protein, [U-98%13C;U-98%15N], 2.5 3.5 mM; D2O 5%; H2O 95%
sample_conditions_one: pH: 4.5; pressure: 1 atm; temperature: 308 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
NOESY | not available | not available | not available |
TOCSY | not available | not available | not available |
DQ-correlation | not available | not available | not available |
DQF-COSY | not available | not available | not available |
3D 15N HSQC-NOESY | not available | not available | not available |
3D 13C HSQC-NOESY | not available | not available | not available |
HCCH | not available | not available | not available |
Software:
FELIX v2.30 - processing and analysis
NMR spectrometers:
- Bruker AMX500 500 MHz
- Bruker DMX600 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts