BMRB Entry 5078
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5078
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Title: Structure and Backbone Dynamics of a Lipoyl Domain from Human Mitochondrial Branched-Chain alpha-Ketoacid Dehydrogenase PubMed: 11839747
Deposition date: 2001-07-12 Original release date: 2004-02-19
Authors: Chang, Chi-Fon; Chou, Hui-Ting; Chuang, Jacinta; Chuang, David; Huang, Tai-huang
Citation: Chang, Chi-Fon; Chou, Hui-Ting; Chuang, Jacinta; Chuang, David; Huang, Tai-huang. "Solution Structure and Dynamics of the Lipoic Acid-bearing Domain of Human Mitochondrial Branched-chain Alpha-Keto Acid Dehydrogenase" J. Biol. Chem. 277, 15865-15873 (2002).
Assembly members:
Lipoyl acide bearing domain, polymer, 93 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Lipoyl acide bearing domain: MGQVVQFKLSDIGEGIREVT
VKEWYVKEGDTVSQFDSICE
VQSDKASVTITSRYDGVIKK
LYYNLDDIAYVGKPLVDIET
EALKDLEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 540 |
| 13C chemical shifts | 345 |
| 15N chemical shifts | 85 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | LBD of human BCKD | 1 |
Entities:
Entity 1, LBD of human BCKD 93 residues - Formula weight is not available
| 1 | MET | GLY | GLN | VAL | VAL | GLN | PHE | LYS | LEU | SER | ||||
| 2 | ASP | ILE | GLY | GLU | GLY | ILE | ARG | GLU | VAL | THR | ||||
| 3 | VAL | LYS | GLU | TRP | TYR | VAL | LYS | GLU | GLY | ASP | ||||
| 4 | THR | VAL | SER | GLN | PHE | ASP | SER | ILE | CYS | GLU | ||||
| 5 | VAL | GLN | SER | ASP | LYS | ALA | SER | VAL | THR | ILE | ||||
| 6 | THR | SER | ARG | TYR | ASP | GLY | VAL | ILE | LYS | LYS | ||||
| 7 | LEU | TYR | TYR | ASN | LEU | ASP | ASP | ILE | ALA | TYR | ||||
| 8 | VAL | GLY | LYS | PRO | LEU | VAL | ASP | ILE | GLU | THR | ||||
| 9 | GLU | ALA | LEU | LYS | ASP | LEU | GLU | HIS | HIS | HIS | ||||
| 10 | HIS | HIS | HIS |
Samples:
sample_1: Lipoyl acide bearing domain, [U-13C; U-15N], 2 – 3 mM
cond_1: pH: 7.5; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY | not available | not available | not available |
| 1H-15N TOCSY | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCO | not available | not available | not available |
| HN(CA)CO | not available | not available | not available |
| CBCANH | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| GB | AAA59200 KFO26886 |
| REF | XP_004385775 XP_005339742 XP_005542667 XP_007123933 XP_007123934 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts