BMRB Entry 5824
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5824
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Title: Sequence-specific 1H, 13C and 15N resonance assignments of the C-terminal domain of KaiA, a circadian clock protein (oxidized form) PubMed: 14872133
Deposition date: 2003-06-08 Original release date: 2004-02-13
Authors: Vakonakis, Ioannis; LiWang, Andy
Citation: Vakonakis, Ioannis; LiWang, Andy. "Letter to the Editor: Sequence-specific 1H, 13C and 15N resonance assignments of the C-terminal domain of KaiA, a circadian clock protein " J. Biomol. NMR 28, 403-404 (2004).
Assembly members:
Thermosynechococcus KaiA, polymer, 107 residues, 12583 Da.
Natural source: Common Name: Thermosynechococcus elongatus Taxonomy ID: 146786 Superkingdom: Eubacteria Kingdom: not available Genus/species: Thermosynechococcus elongatus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Thermosynechococcus KaiA: AMARMSPADKRKLLDELRSI
YRTIVLEYFNTDAKVNERID
EFVSKAFFADISVSQVLEIH
VELMDTFSKQLKLEGRSEDI
LLDYRLTLIDVIAHLCEMYR
RSIPREV
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 789 |
13C chemical shifts | 392 |
15N chemical shifts | 99 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | ThKaiA180C subunit 1 | 1 |
2 | ThKaiA180C subunit 2 | 1 |
Entities:
Entity 1, ThKaiA180C subunit 1 107 residues - 12583 Da.
1 | ALA | MET | ALA | ARG | MET | SER | PRO | ALA | ASP | LYS | ||||
2 | ARG | LYS | LEU | LEU | ASP | GLU | LEU | ARG | SER | ILE | ||||
3 | TYR | ARG | THR | ILE | VAL | LEU | GLU | TYR | PHE | ASN | ||||
4 | THR | ASP | ALA | LYS | VAL | ASN | GLU | ARG | ILE | ASP | ||||
5 | GLU | PHE | VAL | SER | LYS | ALA | PHE | PHE | ALA | ASP | ||||
6 | ILE | SER | VAL | SER | GLN | VAL | LEU | GLU | ILE | HIS | ||||
7 | VAL | GLU | LEU | MET | ASP | THR | PHE | SER | LYS | GLN | ||||
8 | LEU | LYS | LEU | GLU | GLY | ARG | SER | GLU | ASP | ILE | ||||
9 | LEU | LEU | ASP | TYR | ARG | LEU | THR | LEU | ILE | ASP | ||||
10 | VAL | ILE | ALA | HIS | LEU | CYS | GLU | MET | TYR | ARG | ||||
11 | ARG | SER | ILE | PRO | ARG | GLU | VAL |
Samples:
sample_1: Thermosynechococcus KaiA, [U-99% 13C; U-99% 15N], 1.2 mM; Na2HPO4 20.0 mM; NaCl 20.0 mM
sample_2: Thermosynechococcus KaiA 1.2 mM; Na2HPO4 20.0 mM; NaCl 20.0 mM
cond_1: pH: 7.0 na; temperature: 323 K; ionic strength: 0.15 M
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
15N HSQC | not available | not available | not available |
13C HSQC | not available | not available | not available |
CBCA(CO)NH | not available | not available | not available |
CBCANH | not available | not available | not available |
HBHA(CO)NH | not available | not available | not available |
C(CO)NH | not available | not available | not available |
H(CCO)NH | not available | not available | not available |
H(C)CH-COSY | not available | not available | not available |
4D 13C-13C edited NOESY | not available | not available | not available |
Software:
NMRPipe v2.1 Rev 2002.044.17.08 - data processing
PIPP v4.2.6 - data analysis
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 500 MHz
Related Database Links:
BMRB | 5825 |
PDB | |
DBJ | BAB85983 BAC08033 BAD21221 |
REF | NP_681271 WP_011056332 |
SP | Q6L8K1 Q79V62 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts