BMRB Entry 6166
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6166
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Title: Solution structure of the second complement control protein (CCP) module of the GABA(B)R1a receptor, Pro-119 cis conformer PubMed: 15304491
Deposition date: 2004-04-02 Original release date: 2004-09-07
Authors: Blein, S.; Uhrin, D.; Smith, B.; White, J.; Barlow, P.
Citation: Blein, S.; Ginham, R.; Uhrin, D.; Smith, B.; Soares, D.; Veltel, S.; McIlhinney, R.A.; White, J.; Barlow, P.. "Structural analysis of the CCP modules of the GABAB receptor 1a: Only one of the two CCP modules is compactly folded." J. Biol. Chem. 279, 48292-48306 (2004).
Assembly members:
Gamma-aminobutyric acid type B receptor, polymer, 68 residues, Formula weight is not available
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: fungal yeast
Entity Sequences (FASTA):
Gamma-aminobutyric acid type B receptor: EAEFVRICSKSYLTLENGKV
FLTGGDLPALDGARVEFRCD
PDFHLVGSSRSVCSQGQWST
PKPHCQVN
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 459 |
| 13C chemical shifts | 230 |
| 15N chemical shifts | 72 |
| coupling constants | 23 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Gamma-aminobutyric acid type B receptor | 1 |
Entities:
Entity 1, Gamma-aminobutyric acid type B receptor 68 residues - Formula weight is not available
| 1 | GLU | ALA | GLU | PHE | VAL | ARG | ILE | CYS | SER | LYS | ||||
| 2 | SER | TYR | LEU | THR | LEU | GLU | ASN | GLY | LYS | VAL | ||||
| 3 | PHE | LEU | THR | GLY | GLY | ASP | LEU | PRO | ALA | LEU | ||||
| 4 | ASP | GLY | ALA | ARG | VAL | GLU | PHE | ARG | CYS | ASP | ||||
| 5 | PRO | ASP | PHE | HIS | LEU | VAL | GLY | SER | SER | ARG | ||||
| 6 | SER | VAL | CYS | SER | GLN | GLY | GLN | TRP | SER | THR | ||||
| 7 | PRO | LYS | PRO | HIS | CYS | GLN | VAL | ASN |
Samples:
sample_1: Gamma-aminobutyric acid type B receptor, [U-15N; U-13C], 1 mM; deuterated sodium acetate 20 mM; D2O 10%; H2O 90%
sample_cond_1: pH: 4; temperature: 310 K; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | sample_cond_1 |
| 3D 15N-separated NOESY | sample_1 | not available | sample_cond_1 |
| HNHA | sample_1 | not available | sample_cond_1 |
Software:
VNMR v6.1 - collection
AZARA v2.6 - processing
ANSIG v3.3 - data analysis
CNS v1.0 - structure solution, refinement
NMR spectrometers:
- Bruker DRX 800 MHz
Related Database Links:
| BMRB | 6171 |
| PDB | |
| EMBL | CAA71398 CAE84069 CAH40831 CAH40832 CAL91172 |
| GB | AAD19656 AAD19658 AAK69540 AAL26807 EDL84628 |
| REF | NP_112290 XP_006255941 XP_008770922 |
| SP | Q9Z0U4 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts