BMRB Entry 6238
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6238
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Complete 1H, 15N amd 13C Chemical Shift Assignments of the murine CBP ZZ domain (a.a. 1700-1751) PubMed: 15476823
Deposition date: 2004-06-14 Original release date: 2004-11-12
Authors: Legge, Glen; Hambly, David; Trinh, Tam; Martinez-Yamout, Maria; Dyson, H.; Wright, Peter
Citation: Legge, Glen; Martinez-Yamout, Maria; Hambly, David; Trinh, Tam; Lee, B.; Dyson, H.; Wright, Peter. "ZZ Domain of CBP: an Unusual Zinc Finger Fold in a Protein Interaction Module" J. Mol. Biol. 343, 1081-1093 (2004).
Assembly members:
ZZ, polymer, 52 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ZZ: GQDRFVYTCNECKHHVETRW
HCTVCEDYDLCINCYNTKSH
THKMVKWGLGLD
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 222 |
1H chemical shifts | 353 |
15N chemical shifts | 56 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | ZZ domain of CBP | 1 |
2 | ZINC (II) ION, 1 | 2 |
3 | ZINC (II) ION, 2 | 2 |
Entities:
Entity 1, ZZ domain of CBP 52 residues - Formula weight is not available
1 | GLY | GLN | ASP | ARG | PHE | VAL | TYR | THR | CYS | ASN | ||||
2 | GLU | CYS | LYS | HIS | HIS | VAL | GLU | THR | ARG | TRP | ||||
3 | HIS | CYS | THR | VAL | CYS | GLU | ASP | TYR | ASP | LEU | ||||
4 | CYS | ILE | ASN | CYS | TYR | ASN | THR | LYS | SER | HIS | ||||
5 | THR | HIS | LYS | MET | VAL | LYS | TRP | GLY | LEU | GLY | ||||
6 | LEU | ASP |
Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.
1 | ZN |
Samples:
sample_1: ZZ, [U-13C; U-15N], 2.3 mM; Zinc Chloride 50 uM; Salt 150 mM; Tris 20 mM; DTT 2 mM; D2O 5 % v/v
Ex-cond_1: pH: 6.8; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1H15N HSQC | sample_1 | not available | Ex-cond_1 |
HNCA | sample_1 | not available | Ex-cond_1 |
CCONH TOCSY | sample_1 | not available | Ex-cond_1 |
HCCONH TOCSY | sample_1 | not available | Ex-cond_1 |
CBCA(CO)NH | sample_1 | not available | Ex-cond_1 |
HCCH TOCSY | sample_1 | not available | Ex-cond_1 |
HCCH COSY | sample_1 | not available | Ex-cond_1 |
HNHB | sample_1 | not available | Ex-cond_1 |
CN NOESY | sample_1 | not available | Ex-cond_1 |
1H/15N NOESY HSQC | sample_1 | not available | Ex-cond_1 |
1H13C HSQC | sample_1 | not available | Ex-cond_1 |
1H13C HSQC aromatic | sample_1 | not available | Ex-cond_1 |
CGCO | sample_1 | not available | Ex-cond_1 |
CGCN | sample_1 | not available | Ex-cond_1 |
CBCGCD | sample_1 | not available | Ex-cond_1 |
HNHA | sample_1 | not available | Ex-cond_1 |
1H15H TOSCY HSQC | sample_1 | not available | Ex-cond_1 |
Software:
NMRPIPE, Ad Bax -
NMRVIEW v5.04, One Moon Scientific -
NMR spectrometers:
- Bruker AMX 500 MHz
- Bruker DRX 600 MHz
Related Database Links:
PDB | |
DBJ | BAE06125 BAI45616 |
GB | AAB28651 AAC17736 AAC51331 AAC51770 AAI72737 |
PRF | 1923401A |
REF | NP_001020603 NP_001073315 NP_001157494 NP_001247644 NP_004371 |
SP | P45481 Q6JHU9 Q92793 |
TPG | DAA15549 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts