BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6315

Title: 1H, 13C and 15N chemical shift assignments for TcUBP1 (GQ) (backbone + side chains)   PubMed: 15751947

Deposition date: 2004-09-09 Original release date: 2005-05-03

Authors: Volpon, Laurent; D'orso, Ivan; Gehring, Kalle

Citation: Volpon, Laurent; D'orso, Ivan; Young, Christopher; Frasch, Alberto; Gehring, Kalle. "NMR structural study of TcUBP1, a single RRM domain protein from Trypanosoma cruzi: contribution of a beta hairpin to RNA binding"  Biochemistry 44, 3708-3717 (2005).

Assembly members:
TcUBP1, polymer, 139 residues, 15413.4 Da.

Natural source:   Common Name: Trypanosoma cruzi   Taxonomy ID: 5693   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Trypanosoma cruzi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
TcUBP1: MSQIPLVSQYDPYGQTAQLQ QLQQQQQQHIPPTQMNPEPD VLRNLMVNYIPTTVDEVQLR QLFERYGPIESVKIVCDRET RQSRGYGFVKFQSGSSAQQA IAGLNGFNILNKRLKVALAA SGHQRPGIAGAVGDGNGYL

Data sets:
Data typeCount
1H chemical shifts757
13C chemical shifts486
15N chemical shifts123

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TcUBP11

Entities:

Entity 1, TcUBP1 139 residues - 15413.4 Da.

1   METSERGLNILEPROLEUVALSERGLNTYR
2   ASPPROTYRGLYGLNTHRALAGLNLEUGLN
3   GLNLEUGLNGLNGLNGLNGLNGLNHISILE
4   PROPROTHRGLNMETASNPROGLUPROASP
5   VALLEUARGASNLEUMETVALASNTYRILE
6   PROTHRTHRVALASPGLUVALGLNLEUARG
7   GLNLEUPHEGLUARGTYRGLYPROILEGLU
8   SERVALLYSILEVALCYSASPARGGLUTHR
9   ARGGLNSERARGGLYTYRGLYPHEVALLYS
10   PHEGLNSERGLYSERSERALAGLNGLNALA
11   ILEALAGLYLEUASNGLYPHEASNILELEU
12   ASNLYSARGLEULYSVALALALEUALAALA
13   SERGLYHISGLNARGPROGLYILEALAGLY
14   ALAVALGLYASPGLYASNGLYTYRLEU

Samples:

sample_1: TcUBP1, [U-13C; U-15N], 1.5 mM; Tris 50 mM; NaCl 50 mM; DTT 1 mM; NaN3 1 mM

sample_conditions: pH: 7.2; temperature: 298 K; pressure: 1 atm; ionic strength: 0.05 M

Experiments:

NameSampleSample stateSample conditions
not availablesample_1not availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
GB AAK53819 AAM74138 AAP42139 EAN98684 EAN98700
REF XP_820535 XP_820551

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts