BMRB Entry 6350
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6350
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Title: 1H, 13C and 15N Chemical Shift Assignments for the LH1 Beta Polypeptide of Rhodospirillum rubrum PubMed: 15740753
Deposition date: 2004-10-12 Original release date: 2005-03-17
Authors: Wang, Zheng-Yu; Gokan, Kazutaka; Kobayashi, Masayuki; Nozawa, Tsunenori
Citation: Wang, Zheng-Yu; Gokan, Kazutaka; Kobayashi, Masayuki; Nozawa, Tsunenori. "Solution Structures of the Core Light-harvesting alpha and beta Polypeptides from Rhodospirillum rubrum: Implications for the Pigment-Protein and Protein-Protein Interactions" J. Mol. Biol. 347, 465-477 (2005).
Assembly members:
Beta subunit of LH1 complex, polymer, 55 residues, Formula weight is not available
Natural source: Common Name: Rhodospirillum rubrum Taxonomy ID: 1085 Superkingdom: Eubacteria Kingdom: not available Genus/species: Rhodospirillum rubrum
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
Beta subunit of LH1 complex: AEVKQESLSGITEGEAKEFH
KIFTSSILVFFGVAAFAHLL
VWIWRPWVPGPNGYS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 332 |
| 13C chemical shifts | 154 |
| 15N chemical shifts | 51 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | LH1 Beta | 1 |
Entities:
Entity 1, LH1 Beta 55 residues - Formula weight is not available
| 1 | ALA | GLU | VAL | LYS | GLN | GLU | SER | LEU | SER | GLY | ||||
| 2 | ILE | THR | GLU | GLY | GLU | ALA | LYS | GLU | PHE | HIS | ||||
| 3 | LYS | ILE | PHE | THR | SER | SER | ILE | LEU | VAL | PHE | ||||
| 4 | PHE | GLY | VAL | ALA | ALA | PHE | ALA | HIS | LEU | LEU | ||||
| 5 | VAL | TRP | ILE | TRP | ARG | PRO | TRP | VAL | PRO | GLY | ||||
| 6 | PRO | ASN | GLY | TYR | SER |
Samples:
sample_LH1b: Beta subunit of LH1 complex, [U-95% 13C; U-95% 15N], 0.5 – 1.5 mM; chloroform-d:methanol-d3 [1:1 (v/v)] 100%
Ex-cond: pH*: . na; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY-HSQC | not available | not available | not available |
| 1H-15N TOCSY-HSQC | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| CBCANH | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HBHANH | not available | not available | not available |
| HBHA(CO)NH | not available | not available | not available |
Software:
NMRView v5.0.4 -
NMR spectrometers:
- Bruker DRX 400 MHz
Related Database Links:
| PDB | |
| GB | AAA26461 ABC23772 AEO49512 |
| REF | WP_011390725 YP_006049309 YP_428059 |
| SP | P0C190 Q2RQ23 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts