BMRB Entry 6355
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6355
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Title: Solution Structure Of The Staphylococcus Epidermis Protein SE0936. Northest Strucutral Genomics Consortium Target SeR8.
Deposition date: 2004-10-14 Original release date: 2006-04-06
Authors: Baran, M.; Huang, Y.; Acton, T.; Xiao, R.; Montelione, G.
Citation: Baran, M.; Huang, Y.; Acton, T.; Xiao, R.; Montelione, G.. "Solution Structure Of The Staphylococcus Epidermis Protein SE0936. Northest Strucutral Genomics Consortium Target SeR8. " . ., .-..
Assembly members:
SE0936, polymer, 88 residues, Formula weight is not available
Natural source: Common Name: Staphylococcus epidermidis Taxonomy ID: 1282 Superkingdom: Bacteria Kingdom: not available Genus/species: Staphylococcus epidermidis
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
SE0936: MPTENPTMFDQVAEVIERLR
PFLLRDGGDCTLVDVEDGIV
KLQLHGACGTCPSSTITLKA
GIERALHEEVPGVIEVEQVF
LEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 573 |
| 13C chemical shifts | 348 |
| 15N chemical shifts | 82 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SE0936 | 1 |
Entities:
Entity 1, SE0936 88 residues - Formula weight is not available
| 1 | MET | PRO | THR | GLU | ASN | PRO | THR | MET | PHE | ASP | ||||
| 2 | GLN | VAL | ALA | GLU | VAL | ILE | GLU | ARG | LEU | ARG | ||||
| 3 | PRO | PHE | LEU | LEU | ARG | ASP | GLY | GLY | ASP | CYS | ||||
| 4 | THR | LEU | VAL | ASP | VAL | GLU | ASP | GLY | ILE | VAL | ||||
| 5 | LYS | LEU | GLN | LEU | HIS | GLY | ALA | CYS | GLY | THR | ||||
| 6 | CYS | PRO | SER | SER | THR | ILE | THR | LEU | LYS | ALA | ||||
| 7 | GLY | ILE | GLU | ARG | ALA | LEU | HIS | GLU | GLU | VAL | ||||
| 8 | PRO | GLY | VAL | ILE | GLU | VAL | GLU | GLN | VAL | PHE | ||||
| 9 | LEU | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: SE0936, [U-5% 13C; U-100% 15N], 8 mM; NaN3 0.02%; DTT 10 mM; CaCl2 5 mM; NaCl 100 mM; MES 20 mM; D2O 5%; H20 95%
sample_2: SE0936, [U-100% 13C; U-100% 15N], 8 mM; NaN3 0.02%; DTT 10 mM; CaCl2 5 mM; NaCl 100 mM; MES 20 mM; D2O 5%; H20 95%
sample_cond_1: pH: 6.5; temperature: 293 K; ionic strength: 100 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| C13-HSQC | not available | not available | not available |
| NH-HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCAB | not available | not available | not available |
| N15-NOESY | not available | not available | not available |
| C13-NOESY | not available | not available | not available |
Software:
X-PLOR v2.9.7 - structure solution, refinement
NMRPipe v2.1 - processing
AutoAssign v1.14 - data analysis
AutoStructure v2.0 - data analysis
SPINS v5.0 - data analysis
HYPER v3.2 - data analysis
sparky v3.106 - data analysis
VNMR v6.1 - collection
xwin-nmr - collection
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAE05324 |
| EMBL | CCB54437 CDI72254 CDM13169 |
| GB | AAO04227 AAW37908 AAW53911 ADC88025 AGC91125 |
| REF | NP_764185 WP_001831958 WP_002433501 WP_002456117 WP_010956550 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts