BMRB Entry 6446
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6446
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Title: Structural and Functional Characterization of Transmembrane Segment IV of the NHE1 Isoform of the Na+/H+ Exchanger PubMed: 15677483
Deposition date: 2004-12-28 Original release date: 2005-06-02
Authors: Slepkov, E.; Rainey, J.; Li, X.; Liu, Y.; Cheng, F.; Lindhout, D.; Sykes, B.; Fliegel, L.
Citation: Slepkov, E.; Rainey, J.; Li, X.; Liu, Y.; Cheng, F.; Lindhout, D.; Sykes, B.; Fliegel, L.. "The Na+/H+ Exchanger isoform 1" J. Biol. Chem. 280, 17863-17872 (2005).
Assembly members:
Sodium/hydrogen exchanger 1, polymer, 27 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Sodium/hydrogen exchanger 1: FLQSDVFFLFLLPPIILDAG
YFLPLRX
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 135 |
| 13C chemical shifts | 52 |
| 15N chemical shifts | 25 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sodium/hydrogen exchanger 1 TM segment IV | 1 |
Entities:
Entity 1, Sodium/hydrogen exchanger 1 TM segment IV 27 residues - Formula weight is not available
| 1 | PHE | LEU | GLN | SER | ASP | VAL | PHE | PHE | LEU | PHE | ||||
| 2 | LEU | LEU | PRO | PRO | ILE | ILE | LEU | ASP | ALA | GLY | ||||
| 3 | TYR | PHE | LEU | PRO | LEU | ARG | HSL |
Samples:
sample_1: Sodium/hydrogen exchanger 1 2 mM; DSS 1 mM; CDCl3 44%; CD3OH 44%; H2O 11%
sample_2: Sodium/hydrogen exchanger 1, [U-15N], 2 mM; DSS 1 mM; CDCl3 44%; CD3OH 44%; H2O 11%
sample_cond_1: pH: .; temperature: 303 K; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | not available | not available | sample_cond_1 |
| HNHA | not available | not available | sample_cond_1 |
| 2D NOESY | not available | not available | sample_cond_1 |
| 2D TOCSY | not available | not available | sample_cond_1 |
Software:
VNMR vvarious - collection
NMRPipe v2.3 - processing
SPARKY v3.109 and 3.110 - data analysis
CNS v1.1 - structure solution, refinement
NMR spectrometers:
- Varian INOVA 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAE02258 BAE29332 BAE32882 BAE38221 BAG36869 |
| EMBL | CAA48771 |
| GB | AAA92976 AAA98479 AAB59460 AAC60606 AAF21350 |
| REF | NP_001233682 NP_001273957 NP_003038 NP_036784 NP_058677 |
| SP | P19634 P26431 P48761 Q61165 |
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