BMRB Entry 6474
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6474
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: The 15N relaxation data and backbone dynamics results of CLV1 pT868 bound KI-FHA from KAPP PubMed: 16042389
Deposition date: 2005-01-30 Original release date: 2005-09-08
Authors: Ding, Zhaofeng; Lee, Gui-in; Liang, Xiangyang; Gallazzi, Fabio; Van Doren, Steven
Citation: Ding, Zhaofeng; Lee, Gui-In; Liang, Xiangyang; Gallazzi, Fabio; Arunima, A.; Van Doren, Steven. "PhosphoThr Peptide Binding Globally Rigidifies Much of the FHA Domain from Arabidopsis Receptor Kinase-Associated Protein Phosphatase" Biochemistry 44, 10119-10134 (2005).
Assembly members:
Kinase Interacting FHA (KI-FHA) domain from KAPP, polymer, 139 residues, 13238 Da.
CLV1 pT868 peptide, polymer, 13 residues, Formula weight is not available
Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Kinase Interacting FHA (KI-FHA) domain from KAPP: GPLGSSWLFLEVIAGPAIGL
QHAVNSTSSSKLPVKLGRVS
PSDLALKDSEVSGKHAQITW
NSTKFKWELVDMGSLNGTLV
NSHSISHPDLGSRKWGNPVE
LASDDIITLGTTTKVYVRIS
SQNEFQIPFKIGVASDPMA
CLV1 pT868 peptide: PEYAYTLKVDEKS
- assigned_chemical_shifts
- order_parameters
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
- heteronucl_NOEs
| Data type | Count |
| T1 relaxation values | 208 |
| T2 relaxation values | 212 |
| order parameters | 101 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Kinase-interacting FHA domain of KAPP | 1 |
| 2 | CLV1 pT868 peptide | 2 |
Entities:
Entity 1, Kinase-interacting FHA domain of KAPP 139 residues - 13238 Da.
| 1 | GLY | PRO | LEU | GLY | SER | SER | TRP | LEU | PHE | LEU | ||||
| 2 | GLU | VAL | ILE | ALA | GLY | PRO | ALA | ILE | GLY | LEU | ||||
| 3 | GLN | HIS | ALA | VAL | ASN | SER | THR | SER | SER | SER | ||||
| 4 | LYS | LEU | PRO | VAL | LYS | LEU | GLY | ARG | VAL | SER | ||||
| 5 | PRO | SER | ASP | LEU | ALA | LEU | LYS | ASP | SER | GLU | ||||
| 6 | VAL | SER | GLY | LYS | HIS | ALA | GLN | ILE | THR | TRP | ||||
| 7 | ASN | SER | THR | LYS | PHE | LYS | TRP | GLU | LEU | VAL | ||||
| 8 | ASP | MET | GLY | SER | LEU | ASN | GLY | THR | LEU | VAL | ||||
| 9 | ASN | SER | HIS | SER | ILE | SER | HIS | PRO | ASP | LEU | ||||
| 10 | GLY | SER | ARG | LYS | TRP | GLY | ASN | PRO | VAL | GLU | ||||
| 11 | LEU | ALA | SER | ASP | ASP | ILE | ILE | THR | LEU | GLY | ||||
| 12 | THR | THR | THR | LYS | VAL | TYR | VAL | ARG | ILE | SER | ||||
| 13 | SER | GLN | ASN | GLU | PHE | GLN | ILE | PRO | PHE | LYS | ||||
| 14 | ILE | GLY | VAL | ALA | SER | ASP | PRO | MET | ALA |
Entity 2, CLV1 pT868 peptide 13 residues - Formula weight is not available
| 1 | PRO | GLU | TYR | ALA | TYR | THR | LEU | LYS | VAL | ASP | ||||
| 2 | GLU | LYS | SER |
Samples:
sample_one: Kinase Interacting FHA (KI-FHA) domain from KAPP, [U-15N], 0.53 mM; CLV1 pT868 peptide 2.1 mM
condition_one: pH: 6.3; temperature: 295 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N T1 relaxation | sample_one | not available | condition_one |
| 15N T2 relaxation | sample_one | not available | condition_one |
| heteronuclear 1H-15N NOE | sample_one | not available | condition_one |
| 1H-15N HSQC | sample_one | not available | condition_one |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 500 MHz
- Varian INOVA 600 MHz