BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6510

Title: Structure and inter-domain interactions of domain II from the blood stage malarial protein, apical membrane antigen 1   PubMed: 15964019

Deposition date: 2005-02-17 Original release date: 2005-08-22

Authors: Feng, Zhi-Ping; Keizer, David; Stevenson, Rachel; Yao, Shenggen; Babon, Jeffrey; Murphy, Vince; Anders, Robin; Norton, Raymond

Citation: Feng, Zhi-Ping; Keizer, David; Stevenson, Rachel; Yao, Shenggen; Babon, Jeffrey; Murphy, Vince; Anders, Robin; Norton, Raymond. "Structure and Inter-domain Interactions of Domain II from the Blood-stage Malarial Protein, Apical Membrane Antigen 1 "  J. Mol. Biol. 350, 641-656 (2005).

Assembly members:
Apical membrane antigen ! Domain II, polymer, 140 residues, Formula weight is not available

Natural source:   Common Name: Plasmodium falciparum   Taxonomy ID: 5833   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Plasmodium falciparum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Apical membrane antigen ! Domain II: MRGSHHHHHHGSNAKFGLWV DGNCEDIPHVNEFPAIDLFE CNKLVFELSASDQPKQYEQH LTDYEKIKEGFKNKNASMIK SAFLPTGAFKADRYKSHGKG YNWGNYNTETQKCEIFNVKP TCLINNSSYIATTALSHPIE

Data sets:
Data typeCount
1H chemical shifts702
13C chemical shifts483
15N chemical shifts140

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AMA1 DII1

Entities:

Entity 1, AMA1 DII 140 residues - Formula weight is not available

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERASNALALYSPHEGLYLEUTRPVAL
3   ASPGLYASNCYSGLUASPILEPROHISVAL
4   ASNGLUPHEPROALAILEASPLEUPHEGLU
5   CYSASNLYSLEUVALPHEGLULEUSERALA
6   SERASPGLNPROLYSGLNTYRGLUGLNHIS
7   LEUTHRASPTYRGLULYSILELYSGLUGLY
8   PHELYSASNLYSASNALASERMETILELYS
9   SERALAPHELEUPROTHRGLYALAPHELYS
10   ALAASPARGTYRLYSSERHISGLYLYSGLY
11   TYRASNTRPGLYASNTYRASNTHRGLUTHR
12   GLNLYSCYSGLUILEPHEASNVALLYSPRO
13   THRCYSLEUILEASNASNSERSERTYRILE
14   ALATHRTHRALALEUSERHISPROILEGLU

Samples:

sample_1: Apical membrane antigen ! Domain II, [U-95% 13C; U-90% 15N], 0.1 – 0.8 mM

Ex-cond_1: pH: 4.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

xwinnmr v3.5 - Data collection, processing

xeasy v1,3 - Data analysis

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker Avance 500 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAM85358 BAM85364 BAM85367 BAM85373 BAM85379
EMBL CAB97181 CAB97184 CAB97187 CAB97189 CAB97193
GB AAA29475 AAA29476 AAA29719 AAB36701 AAB50405
REF XP_001348015
SP P22621 P50490

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts