BMRB Entry 6536
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6536
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Title: 1H,15N,13C backbone and sidechain assignment for human ADAP hSH3-1 domain in reduced form
Deposition date: 2005-03-07 Original release date: 2015-05-08
Authors: Zimmermann, Juergen; Freund, Christian
Citation: Zimmermann, Juergen; Freund, Christian. "NMR assignment of the reduced and oxidized forms of the human ADAP hSH3-1 domain" J. Biomol. NMR 32, 94-94 (2005).
Assembly members:
hSH3-1 domain, polymer, 96 residues, 11861.6 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
hSH3-1 domain: EKKEQKEKEKKEQEIKKKFK
LTGPIQVIHLAKACCDVKGG
KNELSFKQGEQIEIIRITDN
PEGKWLGRTARGSYGYIKTT
AVEIDYDSLKLKKDLE
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 239 |
15N chemical shifts | 85 |
1H chemical shifts | 479 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | hSH3-1 domain | 1 |
Entities:
Entity 1, hSH3-1 domain 96 residues - 11861.6 Da.
1 | GLU | LYS | LYS | GLU | GLN | LYS | GLU | LYS | GLU | LYS | ||||
2 | LYS | GLU | GLN | GLU | ILE | LYS | LYS | LYS | PHE | LYS | ||||
3 | LEU | THR | GLY | PRO | ILE | GLN | VAL | ILE | HIS | LEU | ||||
4 | ALA | LYS | ALA | CYS | CYS | ASP | VAL | LYS | GLY | GLY | ||||
5 | LYS | ASN | GLU | LEU | SER | PHE | LYS | GLN | GLY | GLU | ||||
6 | GLN | ILE | GLU | ILE | ILE | ARG | ILE | THR | ASP | ASN | ||||
7 | PRO | GLU | GLY | LYS | TRP | LEU | GLY | ARG | THR | ALA | ||||
8 | ARG | GLY | SER | TYR | GLY | TYR | ILE | LYS | THR | THR | ||||
9 | ALA | VAL | GLU | ILE | ASP | TYR | ASP | SER | LEU | LYS | ||||
10 | LEU | LYS | LYS | ASP | LEU | GLU |
Samples:
sample_1: SH3 domain, [U-13C; U-15N], 1.0 ± 0.05 mM; NaCl 20 mM; Potassiumdihydrogenphosphate 50 mM; Dithtiothreitol 10 mM
conditions_1: pH: 7.0; temperature: 300 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
15N_NOESY | sample_1 | isotropic | conditions_1 |
13C_NOESY | sample_1 | isotropic | conditions_1 |
HNCO | sample_1 | isotropic | conditions_1 |
2D_NOESY | sample_1 | isotropic | conditions_1 |
15N_HSQC | sample_1 | isotropic | conditions_1 |
Software:
AZARA v2.0 - data processing
ANSIG v3.3 - assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts