BMRB Entry 6559
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6559
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Title: Structure of the FBP11WW1 domain complexed to the peptide APPTPPPLPP PubMed: 15811376
Deposition date: 2005-03-18 Original release date: 2005-08-22
Authors: Pires, J.; Parthier, C.; Aido-Machado, R.; Wiedemann, U.; Otte, L.; Boehm, G.; Rudolph, R.; Oschkinat, H.
Citation: Pires, J.; Parthier, C.; Aido-Machado, R.; Wiedemann, U.; Otte, L.; Boehm, G.; Rudolph, R.; Oschkinat, H.. "Structural basis for APPTPPPLPP peptide recognition by the FBP11WW1 domain" J. Mol. Biol. 348, 399-408 (2005).
Assembly members:
Formin-binding protein 3, polymer, 41 residues, Formula weight is not available
Formin, polymer, 10 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Formin-binding protein 3: GSRRASVGSAKSMWTEHKSP
DGRTYYYNTETKQSTWEKPD
D
Formin: APPTPPPLPP
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 483 |
| 13C chemical shifts | 122 |
| 15N chemical shifts | 42 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Formin-binding protein 3 | 1 |
| 2 | Formin | 2 |
Entities:
Entity 1, Formin-binding protein 3 41 residues - Formula weight is not available
| 1 | GLY | SER | ARG | ARG | ALA | SER | VAL | GLY | SER | ALA | ||||
| 2 | LYS | SER | MET | TRP | THR | GLU | HIS | LYS | SER | PRO | ||||
| 3 | ASP | GLY | ARG | THR | TYR | TYR | TYR | ASN | THR | GLU | ||||
| 4 | THR | LYS | GLN | SER | THR | TRP | GLU | LYS | PRO | ASP | ||||
| 5 | ASP |
Entity 2, Formin 10 residues - Formula weight is not available
| 1 | ALA | PRO | PRO | THR | PRO | PRO | PRO | LEU | PRO | PRO |
Samples:
sample_1: Formin-binding protein 3, [U-15N; U-13C], 1.8 mM; Formin 3.6 mM; phosphate buffer 10 mM; Nacl 100 mM; DTT 0.1 mM; EDTA 0.1 mM; H2O 90%; D2O 10%
sample_2: Formin-binding protein 3, [U-15N; U-13C], 1.8 mM; Formin 3.6 mM; phosphate buffer 10 mM; Nacl 100 mM; DTT 0.1 mM; EDTA 0.1 mM; D2O 100%
sample_3: Formin-binding protein 3, [U-15N], 1.8 mM; Formin 3.6 mM; phosphate buffer 10 mM; Nacl 100 mM; DTT 0.1 mM; EDTA 0.1 mM; H2O 90%; D2O 10%
sample_4: Formin-binding protein 3 1.8 mM; Formin 3.6 mM; phosphate buffer 10 mM; Nacl 100 mM; DTT 0.1 mM; EDTA 0.1 mM; H2O 90%; D2O 10%
sample_cond_1: pH: 6.0; temperature: 298 K; ionic strength: 100 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| triple-resonance | not available | not available | not available |
| 3D 13C-separated NOESY | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
Software:
CNS v1.1 - refinement
XWINNMR v3.2 - collection, processing
Sparky v3.1 - data analysis
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| SWISS-PROT | O75400 |
| REF | XP_001926823 XP_001916024 XP_001508338 XP_001365548 NP_060362 |
| GenBank | AAH11788 AAC27506 AAC27502 AAC27501 AAB93495 |
| DBJ | BAB15016 |
| PDB | |
| BMRB | 6721 6719 6558 |
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