BMRB Entry 6612
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6612
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Title: NMR structure of unliagnded MDM2 PubMed: 15953616
Deposition date: 2005-04-28 Original release date: 2005-10-27
Authors: Uhrinova, S.; Uhrin, D.; Powers, H.; Watt, K.; Zheleva, D.; Fischer, P.; McInnes, C.; Barlow, P.
Citation: Uhrinova, S.; Uhrin, D.; Powers, H.; Watt, K.; Zheleva, D.; Fischer, P.; McInnes, C.; Barlow, P.. "Structure of free MDM2 N-terminal domain reveals conformational adjustments that accompany p53-binding." J. Mol. Biol. 350, 587-598 (2005).
Assembly members:
Ubiquitin-protein ligase E3 Mdm2 (E.C.6.3.2.-), polymer, 119 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Ubiquitin-protein ligase E3 Mdm2 (E.C.6.3.2.-): MCNTNMSVPTDGAVTTSQIP
ASEQETLVRPKPLLLKLLKS
VGAQKDTYTMKEVLFYLGQY
IMTKRLYDEKQQHIVYCSND
LLGDLFGVPSFSVKEHRKIY
TMIYRNLVVVNQQESSDSS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 719 |
| 13C chemical shifts | 320 |
| 15N chemical shifts | 103 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ubiquitin-protein ligase E3 Mdm2 | 1 |
Entities:
Entity 1, Ubiquitin-protein ligase E3 Mdm2 119 residues - Formula weight is not available
| 1 | MET | CYS | ASN | THR | ASN | MET | SER | VAL | PRO | THR | ||||
| 2 | ASP | GLY | ALA | VAL | THR | THR | SER | GLN | ILE | PRO | ||||
| 3 | ALA | SER | GLU | GLN | GLU | THR | LEU | VAL | ARG | PRO | ||||
| 4 | LYS | PRO | LEU | LEU | LEU | LYS | LEU | LEU | LYS | SER | ||||
| 5 | VAL | GLY | ALA | GLN | LYS | ASP | THR | TYR | THR | MET | ||||
| 6 | LYS | GLU | VAL | LEU | PHE | TYR | LEU | GLY | GLN | TYR | ||||
| 7 | ILE | MET | THR | LYS | ARG | LEU | TYR | ASP | GLU | LYS | ||||
| 8 | GLN | GLN | HIS | ILE | VAL | TYR | CYS | SER | ASN | ASP | ||||
| 9 | LEU | LEU | GLY | ASP | LEU | PHE | GLY | VAL | PRO | SER | ||||
| 10 | PHE | SER | VAL | LYS | GLU | HIS | ARG | LYS | ILE | TYR | ||||
| 11 | THR | MET | ILE | TYR | ARG | ASN | LEU | VAL | VAL | VAL | ||||
| 12 | ASN | GLN | GLN | GLU | SER | SER | ASP | SER | SER |
Samples:
sample_1: Ubiquitin-protein ligase E3 Mdm2 (E.C.6.3.2.-), [U-13C; U-15N], 0.5 mM; sodium acetate, [U-2H], 60 mM; phosphate buffer 60 mM; H2O 90%; D2O 10%
sample_2: Ubiquitin-protein ligase E3 Mdm2 (E.C.6.3.2.-), [U-13C; U-15N], 0.5 mM; sodium acetate, [U-2H], 60 mM; phosphate buffer 60 mM; D2O 99.5%
sample_cond_1: pH: 7.3; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
AZARA - processing
ANSIG - data analysis
CNS - structure solution, refinement
NMR spectrometers:
- Bruker AMX 600 MHz
Related Database Links:
| BMRB | 18755 19230 2410 |
| PDB | |
| DBJ | BAF83030 BAJ17752 |
| EMBL | CAA78055 CAD23251 CAD36959 CAD79457 CAH89564 |
| GB | AAA60568 AAI48523 AAI52385 AAI52391 AAI53118 |
| PRF | 1814460A |
| REF | NP_001124685 NP_001138809 NP_001138811 NP_001253331 NP_002383 |
| SP | Q00987 |
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