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Biological Magnetic Resonance Data Bank


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BMRB Entry 6635

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6635
MolProbity Validation Chart

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Title: Backbone 1H, 13C, and 15N Resonance Assignments for the 13kD Ras Association Domain 2 (RA2) from Phospholipase C (epsilon)   PubMed: 16258837

Deposition date: 2005-05-16 Original release date: 2005-11-07

Authors: Harris, Richard; Bunney, Tom; Katan, Matilda; Driscoll, Paul

Citation: Harris, Richard; Bunney, Tom; Katan, Matilda; Driscoll, Paul. "Backbone 1H, 13C, and 15N Resonance Assignments for the two 13 kD Ras Associating Domains (RA1 and RA2) from Phospholipase C(epsilon)"  J. Biomol. NMR 33, 138-138 (2005).

Assembly members:
ra2, polymer, 117 residues, 13146 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
ra2: GSSEEESFFVQVHDVSPEQP LTVIKAPRVSTAQDVIQQTL CKAKYSYSILSNPNPSDYVL LEEVVKDTTNKKTTTPKSSQ RVLLDQECVFQAQSKWKGAG KFILKLKEQVQASREDK

Data sets:
Data typeCount
13C chemical shifts433
15N chemical shifts96
1H chemical shifts750

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RA21

Entities:

Entity 1, RA2 117 residues - 13146 Da.

additional Glycine at N-terminus

1   GLYSERSERGLUGLUGLUSERPHEPHEVAL
2   GLNVALHISASPVALSERPROGLUGLNPRO
3   LEUTHRVALILELYSALAPROARGVALSER
4   THRALAGLNASPVALILEGLNGLNTHRLEU
5   CYSLYSALALYSTYRSERTYRSERILELEU
6   SERASNPROASNPROSERASPTYRVALLEU
7   LEUGLUGLUVALVALLYSASPTHRTHRASN
8   LYSLYSTHRTHRTHRPROLYSSERSERGLN
9   ARGVALLEULEUASPGLNGLUCYSVALPHE
10   GLNALAGLNSERLYSTRPLYSGLYALAGLY
11   LYSPHEILELEULYSLEULYSGLUGLNVAL
12   GLNALASERARGGLUASPLYS

Samples:

15N: RA2, [U-15N], 1 mM; sodium chloride 250 mM; DTT 5 mM; sodium phosphate 25 mM; EDTA 0.1 mM

13C15N: RA2, [U13C; U-15N], 1 mM; sodium chloride 250 mM; DTT 5 mM; sodium phospahte 25 mM; EDTA 0.1 mM

1H: RA2, unlabeled, 1 mM; sodium chloride 250 mM; DTT 5 mM; sodium phospahte 25 mM; EDTA 0.1 mM; dueterium oxide 100%

conditions_1: pH: 6.5; temperature: 298 K

unlabelled: pD: 6.1; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCnot availablenot availablenot available
13C1H HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNcoCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCAcoNHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HAcacoNHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
13C NOESYnot availablenot availablenot available
15N NOESYnot availablenot availablenot available
NOESYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
HACANHnot availablenot availablenot available

Software:

ANSIG v3.3 - analysing NMR spectra

NMRPipe v2.3 - Processing NMR spectra

AZARA v2.7 - conversion of nmrPipe format data to ansig format

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian UnityPlus 500 MHz

Related Database Links:

PDB
DBJ BAA96040 BAB14090 BAG10469
GB AAF22005 AAG17145 AAG28341 AAI40706 AAI44287
REF NP_001159451 NP_001275918 NP_057425 XP_001502425 XP_002805823
SP Q9P212

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts