BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6714

Title: Solution structure of CSP1   PubMed: 16484185

Deposition date: 2005-06-30 Original release date: 2006-04-24

Authors: Johnsborg, O.; Kristiansen, P.

Citation: Johnsborg, O.; Kristiansen, P.; Blomqvist, T.; Havarstein, L.. "A hydrophobic patch in the competence-stimulating Peptide, a pneumococcal competence pheromone, is essential for specificity and biological activity"  J. Bacteriol. 188, 1744-1749 (2006).

Assembly members:
CSP1, polymer, 17 residues, Formula weight is not available

Natural source:   Common Name: Streptococcus pneumoniae   Taxonomy ID: 1313   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Streptococcus pneumoniae

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
CSP1: EMRLSKFFRDFILQRKK

Data sets:
Data typeCount
1H chemical shifts144

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CSP11

Entities:

Entity 1, CSP1 17 residues - Formula weight is not available

1   GLUMETARGLEUSERLYSPHEPHEARGASP
2   PHEILELEUGLNARGLYSLYS

Samples:

sample_1: CSP1 1.9 mM; TFA 0.1%; D38 DPC 250 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 5.2; temperature: 301 K; ionic strength: 0 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available

Software:

NMRPipe v2004 - processing

SPARKY v2004 - data analysis

CYANA v2004 - refinement

TALOS v2004 - data analysis

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
EMBL CAB39422 CAB39424 CAB39434 CAB39436 CAB39438
GB AAC44440 AAC44895 AAK48419 AAL00845 AAS48490
REF NP_359634 WP_000799689 WP_000799690 WP_000799691 YP_001695587
SP P60242 P60243