BMRB Entry 6801
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6801
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Title: 1H, 13C, and 15N Chemical Shift Assignments for Human Small Ubiquitin-like Modifier Protein Isoform 2 (SUMO-2)
Deposition date: 2005-08-30 Original release date: 2007-04-11
Authors: Chang, Chung-ke; Wang, Ying Hui; Chung, Tung-Liang; Chang, Chi-Fon; Li, Steven; Huang, Tai-huang
Citation: Chang, Chung-ke; Wang, Ying Hui; Chang, Chi-Fon; Chung, Tung-Liang; Tien, Shi-chi; Li, Steven; Huang, Tai-huang. "Solution structure and dynamics of human SUMO-2" J. Mol. Biol. ., .-..
Assembly members:
human SUMO-2, polymer, 96 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
human SUMO-2: MADEKPKEGVKTENNDHINL
KVAGQDGSVVQFKIKRHTPL
SKLMKAYCERQGLSMRQIRF
RFDGQPINETDTPAQLEMED
EDTIDVFQQQTGGVYY
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 366 |
| 15N chemical shifts | 92 |
| 1H chemical shifts | 584 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Human SUMO-2 monomer | 1 |
Entities:
Entity 1, Human SUMO-2 monomer 96 residues - Formula weight is not available
| 1 | MET | ALA | ASP | GLU | LYS | PRO | LYS | GLU | GLY | VAL | ||||
| 2 | LYS | THR | GLU | ASN | ASN | ASP | HIS | ILE | ASN | LEU | ||||
| 3 | LYS | VAL | ALA | GLY | GLN | ASP | GLY | SER | VAL | VAL | ||||
| 4 | GLN | PHE | LYS | ILE | LYS | ARG | HIS | THR | PRO | LEU | ||||
| 5 | SER | LYS | LEU | MET | LYS | ALA | TYR | CYS | GLU | ARG | ||||
| 6 | GLN | GLY | LEU | SER | MET | ARG | GLN | ILE | ARG | PHE | ||||
| 7 | ARG | PHE | ASP | GLY | GLN | PRO | ILE | ASN | GLU | THR | ||||
| 8 | ASP | THR | PRO | ALA | GLN | LEU | GLU | MET | GLU | ASP | ||||
| 9 | GLU | ASP | THR | ILE | ASP | VAL | PHE | GLN | GLN | GLN | ||||
| 10 | THR | GLY | GLY | VAL | TYR | TYR |
Samples:
sample_1: human SUMO-2, [U-95% 13C; U-95% 15N], 0.8 ± 0.1 mM; sodium phoshate 50 ± 1 mM; sodium chloride 150 ± 1 mM; EDTA 1 ± 0.01 mM; DSS 1 ± 0.01 mM; deuterium oxide 10 ± 1 %; water 90 ± 1 %; sodium azide 0.01 ± 0.0001 %; Complete(R) protease inhibitor cocktail
conditions_1: pH: 6.0; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | sample_1 | not available | conditions_1 |
| 1H15N_NOESY_HSQC | sample_1 | not available | conditions_1 |
| HNCA | sample_1 | not available | conditions_1 |
| HN(CO)CA | sample_1 | not available | conditions_1 |
| HNCO | sample_1 | not available | conditions_1 |
| HN(CA)CO | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| CBCA(CO)NH | sample_1 | not available | conditions_1 |
| HCCH_TOCSY | sample_1 | not available | conditions_1 |
| 1H13C_NOESY_HSQC | sample_1 | not available | conditions_1 |
| HBCB(CGCD)HD | sample_1 | not available | conditions_1 |
| HBCB(CGCDCE)HE | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 500 MHz
Related Database Links:
| BMRB | 11267 19961 |
| PDB | |
| DBJ | BAB28360 BAC39397 BAE21037 BAE35772 BAE39412 |
| EMBL | CAA67897 CAG32064 CAL37097 |
| GB | AAB49682 AAB92355 AAD45399 AAH08450 AAH16775 |
| REF | NP_001003422 NP_001074186 NP_001165519 NP_001185620 NP_001231073 |
| SP | P61955 P61956 P61957 P61958 P61959 |
| TPG | DAA13334 DAA18189 |
Download simulated HSQC data in one of the following formats:
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