BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6818

Title: Structure of the cyclic conotoxin MII-6   PubMed: 16162671

Deposition date: 2005-09-09 Original release date: 2005-11-21

Authors: Clark, R.; Fischer, H.; Dempster, L.; Daly, N.; Rosengren, K.; Nevin, S.; Meunier, F.; Adams, D.; Craik, D.

Citation: Clark, R.; Fischer, H.; Dempster, L.; Daly, N.; Rosengren, K.; Nevin, S.; Meunier, F.; Adams, D.; Craik, D.. "Engineering stable peptide toxins by means of backbone cyclization: stabilization of the alpha-conotoxin MII."  Proc. Natl. Acad. Sci. U. S. A. 102, 13767-13772 (2005).

Assembly members:
Alpha-conotoxin MII-6, polymer, 22 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Alpha-conotoxin MII-6: GCCSNPVCHLEHSNLCGGAA GG

Data sets:
Data typeCount
1H chemical shifts99

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Alpha-conotoxin MII-61

Entities:

Entity 1, Alpha-conotoxin MII-6 22 residues - Formula weight is not available

1   GLYCYSCYSSERASNPROVALCYSHISLEU
2   GLUHISSERASNLEUCYSGLYGLYALAALA
3   GLYGLY

Samples:

sample_1: Alpha-conotoxin MII-6 1 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 3.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available
E-COSYnot availablenot availablenot available

Software:

xwinnmr v3.5 - collection

XEASY v1.3.7 - data analysis

DYANA v1.5 - structure solution

CNS v1.1 - refinement, structure solution

NMR spectrometers:

  • Bruker DMX 750 MHz

Related Database Links:

PDB
PRF 2211312A
SWISS-PROT P56636