BMRB Entry 6914
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6914
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of the Wilson ATPase N-domain in the presence of ATP PubMed: 16868859
Deposition date: 2005-12-01 Original release date: 2007-02-09
Authors: Dmitriev, O.; Tsivkovski, R.; Abildgaard, F.; Morgan, C.; Markley, J.; Lutsenko, S.
Citation: Dmitriev, O.; Tsivkovski, R.; Abildgaard, F.; Lutsenko, S.. "NMR assignment of the Wilson disease associated protein N-domain" J. Biomol. NMR 36, 61-61 (2006).
Assembly members:
Wilson Disease ATPase N-domain, polymer, 165 residues, 17292 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Wilson Disease ATPase N-domain: AGHMVPRVMRVLLLGDVATL
PLRKVLAVVGTAEASSEHPL
GVAVTKYCKEELGTETLGYC
TDFQAVPGCGIGCKVSNVEG
ILAHSERPLSAPASHLNEAG
SLPAEKDAVPQTFSVLIGNR
EWLRRNGLTISSDVSDAMTD
HEMKGQTAILVAIDGVLCGM
IAIAD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 645 |
| 15N chemical shifts | 164 |
| 1H chemical shifts | 1019 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | WILSON DISEASE ATPASE, N-domain | 1 |
Entities:
Entity 1, WILSON DISEASE ATPASE, N-domain 165 residues - 17292 Da.
| 1 | ALA | GLY | HIS | MET | VAL | PRO | ARG | VAL | MET | ARG | ||||
| 2 | VAL | LEU | LEU | LEU | GLY | ASP | VAL | ALA | THR | LEU | ||||
| 3 | PRO | LEU | ARG | LYS | VAL | LEU | ALA | VAL | VAL | GLY | ||||
| 4 | THR | ALA | GLU | ALA | SER | SER | GLU | HIS | PRO | LEU | ||||
| 5 | GLY | VAL | ALA | VAL | THR | LYS | TYR | CYS | LYS | GLU | ||||
| 6 | GLU | LEU | GLY | THR | GLU | THR | LEU | GLY | TYR | CYS | ||||
| 7 | THR | ASP | PHE | GLN | ALA | VAL | PRO | GLY | CYS | GLY | ||||
| 8 | ILE | GLY | CYS | LYS | VAL | SER | ASN | VAL | GLU | GLY | ||||
| 9 | ILE | LEU | ALA | HIS | SER | GLU | ARG | PRO | LEU | SER | ||||
| 10 | ALA | PRO | ALA | SER | HIS | LEU | ASN | GLU | ALA | GLY | ||||
| 11 | SER | LEU | PRO | ALA | GLU | LYS | ASP | ALA | VAL | PRO | ||||
| 12 | GLN | THR | PHE | SER | VAL | LEU | ILE | GLY | ASN | ARG | ||||
| 13 | GLU | TRP | LEU | ARG | ARG | ASN | GLY | LEU | THR | ILE | ||||
| 14 | SER | SER | ASP | VAL | SER | ASP | ALA | MET | THR | ASP | ||||
| 15 | HIS | GLU | MET | LYS | GLY | GLN | THR | ALA | ILE | LEU | ||||
| 16 | VAL | ALA | ILE | ASP | GLY | VAL | LEU | CYS | GLY | MET | ||||
| 17 | ILE | ALA | ILE | ALA | ASP |
Samples:
sample_1: Wilson Disease ATPase N-domain, [U-99% 13C; U-95% 15N], 0.75 mM; sodium phosphate 50 mM; DTT 5 mM; NaN3 50 mM; ATP 5 mM; H2O 95%; D2O 5%
sample_cond_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | not available | sample_cond_1 |
| 3D 13C-separated NOESY | sample_1 | not available | sample_cond_1 |
| 4D 13C/15N-separated NOESY | sample_1 | not available | sample_cond_1 |
Software:
xwinnmr v3.5 - collection
FELIX v2000 - data analysis
CYANA v1.0 - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 600 MHz
Related Database Links:
| BMRB | 16761 |
| PDB | |
| DBJ | BAD92698 BAG63463 |
| GB | AAA79212 AAA92667 AAB52902 AAI17201 AAI43974 |
| PIR | S40525 |
| PRF | 2001422A |
| REF | NP_000044 NP_001005918 NP_001230111 XP_001103242 XP_003270167 |
| SP | P35670 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts