BMRB Entry 6925
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6925
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Title: Backbone and Beta 1H, 13C, and 15N Chemical Shift Assignments for the Bacterial Toxin Kid PubMed: 16413033
Deposition date: 2006-04-13 Original release date: 2006-11-06
Authors: Kamphuis, Monique; Boelens, Rolf
Citation: Kamphuis, Monique; Bonvin, A.; Monti, M.; Lemonnier, M.; Munoz-Gomez, A.; van den Heuvel, R.; Diaz-Orejas, R.; Boelens, Rolf. "Model for RNA binding and the catalytic site of the RNase Kid of the bacterial parD toxin-antitoxin system" J. Mol. Biol. 357, 115-126 (2006).
Assembly members:
Kid toxin, polymer, 110 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Kid toxin: MERGEIWLVSLDPTAGHEQQ
GTRPVLIVTPAAFNRVTRLP
VVVPVTSGGNFARTAGFAVS
LDGVGIRTTGVVRCDQPRTI
DMKARGGKRLERVPETIMNE
VLGRLSTILT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 308 |
| 15N chemical shifts | 102 |
| 1H chemical shifts | 384 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Kid toxin | 1 |
Entities:
Entity 1, Kid toxin 110 residues - Formula weight is not available
| 1 | MET | GLU | ARG | GLY | GLU | ILE | TRP | LEU | VAL | SER | |
| 2 | LEU | ASP | PRO | THR | ALA | GLY | HIS | GLU | GLN | GLN | |
| 3 | GLY | THR | ARG | PRO | VAL | LEU | ILE | VAL | THR | PRO | |
| 4 | ALA | ALA | PHE | ASN | ARG | VAL | THR | ARG | LEU | PRO | |
| 5 | VAL | VAL | VAL | PRO | VAL | THR | SER | GLY | GLY | ASN | |
| 6 | PHE | ALA | ARG | THR | ALA | GLY | PHE | ALA | VAL | SER | |
| 7 | LEU | ASP | GLY | VAL | GLY | ILE | ARG | THR | THR | GLY | |
| 8 | VAL | VAL | ARG | CYS | ASP | GLN | PRO | ARG | THR | ILE | |
| 9 | ASP | MET | LYS | ALA | ARG | GLY | GLY | LYS | ARG | LEU | |
| 10 | GLU | ARG | VAL | PRO | GLU | THR | ILE | MET | ASN | GLU | |
| 11 | VAL | LEU | GLY | ARG | LEU | SER | THR | ILE | LEU | THR |
Samples:
sample_1: Kid toxin, [U-13C; U-15N], 0.4 mM; PO4 (K) 100 mM
conditions_1: pH: 5.8; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | not available | conditions_1 |
| 2D 1H-13C HSQC | sample_1 | not available | conditions_1 |
| 3D HNCA | sample_1 | not available | conditions_1 |
| 3D HN(CO)CA | sample_1 | not available | conditions_1 |
| 3D HNCACB | sample_1 | not available | conditions_1 |
| 3D CBA(CO)NH | sample_1 | not available | conditions_1 |
| 3D HNCO | sample_1 | not available | conditions_1 |
| 3D HA(CA)NH | sample_1 | not available | conditions_1 |
| 3D HBA(CO)NH | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker AVANCE 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA78898 BAL45864 BAL48674 BAN28644 BAP10671 |
| EMBL | CAA29585 CAB90820 CAB90822 CAD27776 CAH64731 |
| GB | AAA26070 AAO49558 AAR05736 AAR25128 AAZ03778 |
| REF | NP_052994 NP_862963 NP_957647 WP_000439432 WP_000439433 |
| SP | P13976 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts