BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7000

Title: Solution structure of the conserved hypothetical protein Rv2302 from the bacterium Mycobacterium tuberculosis   PubMed: 16885468

Deposition date: 2006-02-23 Original release date: 2006-03-20

Authors: Buchko, Garry; Kim, Chang; Terwilliger, Thomas; Kennedy, Michael

Citation: Buchko, Garry; Kim, Chang; Terwilliger, Thomas; Kennedy, Michael. "Solution structure of the conserved hypothetical protein Rv2302 from the bacterium Mycobacterium tuberculosis"  J. Bacteriol. 188, 5993-6001 (2006).

Assembly members:
Rv2302, polymer, 83 residues, Formula weight is not available

Natural source:   Common Name: Mycobacterium tuberculosis   Taxonomy ID: 1773   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Mycobacterium tuberculosis

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Rv2302: GSHMHAKVGDYLVVKGTTTE RHDQHAEIIEVRSADGSPPY VVRWLVNGHETTVYPGSDAV VVTATEHAEAEKRAAARAGH AAT

Data sets:
Data typeCount
13C chemical shifts292
15N chemical shifts74
1H chemical shifts460

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rv23021

Entities:

Entity 1, Rv2302 83 residues - Formula weight is not available

1   GLYSERHISMETHISALALYSVALGLYASP
2   TYRLEUVALVALLYSGLYTHRTHRTHRGLU
3   ARGHISASPGLNHISALAGLUILEILEGLU
4   VALARGSERALAASPGLYSERPROPROTYR
5   VALVALARGTRPLEUVALASNGLYHISGLU
6   THRTHRVALTYRPROGLYSERASPALAVAL
7   VALVALTHRALATHRGLUHISALAGLUALA
8   GLULYSARGALAALAALAARGALAGLYHIS
9   ALAALATHR

Samples:

sample_1: Rv2302, [U-13C; U-15N], 2 ± 0.3 mM; KCl, none, 100 ± 2 mM; potassium phosphate, none, 20 ± 1 mM; dithiothreitol, none, 2 ± 0.5 mM

conditions_1: pH: 7.1; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQC_(example)sample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1
CC-TOCSY_NNHsample_1not availableconditions_1
4D CC-NOESY-HMQCsample_1not availableconditions_1
N15edited NOESY-HSQCsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 500 MHz
  • Varian Inova 600 MHz

Related Database Links:

PDB
DBJ BAH26596 BAL66308 BAQ06374 GAA45983
EMBL CAD97185 CAL72306 CCC27379 CCC44660 CCC64895
GB AAK46644 ABQ74085 ABR06654 ACT24737 AEB03820
REF NP_216818 NP_336830 NP_855973 WP_003411866 WP_014001212
SP P64984 P9WLD4 P9WLD5

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts