BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 7012

Title: Solution structure of 55-72 segment of staphylococcal nuclease   PubMed: 16767771

Deposition date: 2006-02-28 Original release date: 2007-11-16

Authors: Wang, M.; Shan, L.; Wang, J.

Citation: Wang, M.; Shan, L.; Wang, J.. "Two peptide fragments G55-I72 and K97-A109 from staphylococcal nuclease exhibit different behaviors in conformational preferences for helix formation"  Biopolymers 83, 268-279 (2006).

Assembly members:
18-mer peptide from Thermonuclease (E.C.3.1.31.1), polymer, 18 residues, Formula weight is not available

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
18-mer peptide from Thermonuclease (E.C.3.1.31.1): GPEASAFTKKMVENAKKI

Data sets:
Data typeCount
13C chemical shifts57
1H chemical shifts108

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
118-mer peptide from Thermonuclease1

Entities:

Entity 1, 18-mer peptide from Thermonuclease 18 residues - Formula weight is not available

1   GLYPROGLUALASERALAPHETHRLYSLYS
2   METVALGLUASNALALYSLYSILE

Samples:

sample_1: 18-mer peptide from Thermonuclease (E.C.3.1.31.1) 2.0 mM; DSS 0.02 mM; NaN3 0.01 mM; trifluoroethanol-d4 40%; H2O 60%

sample_cond_1: ionic strength: 0 M; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
2D ROESYnot availablenot availablenot available
2D 13C HSQCnot availablenot availablenot available
2D 13C HSQC-TOCSYnot availablenot availablenot available

Software:

xwinnmr v3.5 - collection

FELIX v98 - data analysis

CNS v1.1 - structure solution, refinement

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 136 15357 1581 1582 1583 1584 15901 16585 1704 17718 18013 1874 1875 1876 1877 1878 18788 188 189 2784 2785 4052 4053 4905 494 495 496 497 530 5536 6250 644 6908
PDB
DBJ BAB94634 BAF67032
EMBL CAA24594 CAG39855 CAG42530 CAI80436 CAQ49298
GB AAC14660 AAW36415 ABD22328 ABD29945 ABE02272
PRF 1109959A 670719A 710414A
REF NP_645586 WP_000141442 WP_000141556 WP_001548082 WP_001566557
SP P00644 Q5HHM4 Q6GB41 Q6GIK1 Q8NXI6