BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules 		Member of WWPDB

BMRB Entry 7178

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR7178
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry

Title: Solution NMR structure of Q8ZP25 from Salmonella typhimurium LT2.

Deposition date: 2006-06-21 Original release date: 2008-01-14

Authors: Parish, D.; Liu, G.; Shen, Y.; Ho, C.; Cunningham, K.; Xiao, R.; Swapna, G.; Acton, T.; Montelione, G.; Szyperski, T.

Citation: Parish, D.; Liu, G.; Shen, Y.; Ho, C.; Cunningham, K.; Xiao, R.; Swapna, G.; Acton, T.; Montelione, G.; Szyperski, T.. "Solution NMR structure of Q8ZP25 from Salmonella typhimurium LT2."  . ., .-..

Assembly members:
putative chaperone, polymer, 142 residues, Formula weight is not available

Natural source:   Common Name: Salmonella typhimurium   Taxonomy ID: 602   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Salmonella typhimurium

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
putative chaperone: MANDTPFSALWQRLLTRGWQ PVEASTVDDWIKRVGDGVIL LSSDPRRTPEVSDNPVMIAE LLREFPQFDWQVAVADLEQS EAIGDRFNVRRFPATLVFTD GKLRGALSGIHPWAELLTLM RSIVDTPAAQETVQLEHHHH HH

Data sets:
Data typeCount
13C chemical shifts356
15N chemical shifts100
1H chemical shifts728

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1putative chaperone1

Entities:

Entity 1, putative chaperone 142 residues - Formula weight is not available

1   METALAASNASPTHRPROPHESERALALEU
2   TRPGLNARGLEULEUTHRARGGLYTRPGLN
3   PROVALGLUALASERTHRVALASPASPTRP
4   ILELYSARGVALGLYASPGLYVALILELEU
5   LEUSERSERASPPROARGARGTHRPROGLU
6   VALSERASPASNPROVALMETILEALAGLU
7   LEULEUARGGLUPHEPROGLNPHEASPTRP
8   GLNVALALAVALALAASPLEUGLUGLNSER
9   GLUALAILEGLYASPARGPHEASNVALARG
10   ARGPHEPROALATHRLEUVALPHETHRASP
11   GLYLYSLEUARGGLYALALEUSERGLYILE
12   HISPROTRPALAGLULEULEUTHRLEUMET
13   ARGSERILEVALASPTHRPROALAALAGLN
14   GLUTHRVALGLNLEUGLUHISHISHISHIS
15   HISHIS

Samples:

sample_1: putative chaperone, [U-13C; U-15N], 1 mM; H20 95%; D20 5%

sample_2: putative chaperone, [U-5% 13C; U-15N], 1 mM; H20 95%; D20 5%

sample_cond_1: pH: 6.5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
Brunger, Adams, Clore, DeLano, Gros, Grosse-Kunstleve, Jiang, Kuszewski, Nilges, Pannu, Read, Rice, Simonson, Warren.not availablenot availablesample_cond_1
Guntertnot availablenot availablesample_cond_1
Variannot availablenot availablesample_cond_1
Delaglio, Baxnot availablenot availablesample_cond_1
Glasernot availablenot availablesample_cond_1
Huang, Tejero, Power, Montelionenot availablenot availablesample_cond_1
Zimmerman, Moseley, Kiriyeva, Kulikowski, Montelionenot availablenot availablesample_cond_1

Software:

CNS v1.1 - refinement

DYANA v1.5 - structure solution

CYANA v1.05 - structure solution

CYANA v2.1 - structure solution

VNMR v6.1c - collection

NMRPipe v2.3 - processing

XEASY v1.3.1.3 - data analysis

AutoStruct v2.0.0 - data analysis

AutoAssign - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAJ36754 BAP07655
EMBL CAD05474 CAR32826 CAR37202 CBG24793 CBW17813
GB AAL20705 AAO68750 ABX66837 ACF61159 ACF68193
PIR AH0721
REF NP_456299 NP_460746 NP_804901 WP_000245855 WP_001262570

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts