BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 7181

Title: Solution Structure of Hypothetical protein PA4359: Northeast Structural Genomics Target PaT89

Deposition date: 2006-06-21 Original release date: 2006-11-08

Authors: Zhang, Q.; Liu, G.; Yee, A.; Arrowsmith, C.; Szyperski, T.

Citation: Zhang, Q.; Liu, G.; Yee, A.; Arrowsmith, C.; Szyperski, T.. "Solution Structure of Hypothetical protein PA4359: Northest Structural Genomics Target PaT89"  . ., .-..

Assembly members:
Hypothetical protein PA4359, polymer, 75 residues, Formula weight is not available

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Hypothetical protein PA4359: MSALQPSRSYRITGYSPAIS NGYRQRLFSMGLLPGAALRV VRIAPLGDPIQVETRQTSLA LRRKDLALLTLVPLD

Data sets:
Data typeCount
13C chemical shifts250
15N chemical shifts67
1H chemical shifts531

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hypothetical protein PA43591

Entities:

Entity 1, Hypothetical protein PA4359 75 residues - Formula weight is not available

1   METSERALALEUGLNPROSERARGSERTYR
2   ARGILETHRGLYTYRSERPROALAILESER
3   ASNGLYTYRARGGLNARGLEUPHESERMET
4   GLYLEULEUPROGLYALAALALEUARGVAL
5   VALARGILEALAPROLEUGLYASPPROILE
6   GLNVALGLUTHRARGGLNTHRSERLEUALA
7   LEUARGARGLYSASPLEUALALEULEUTHR
8   LEUVALPROLEUASP

Samples:

sample_1: Hypothetical protein PA4359, [U-13C; U-15N], 0.7 mM; tris 10 mM; NaCl 300 mM; glycerol 5%; NaN3 0.01%; benzamidiine 1 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
GFT (4,3)D HNNCABCAsample_1not availablesample_cond_1
GFT (4,3)D CABCA(CO)NHNsample_1not availablesample_cond_1
GFT (4,3)D HABCAB(CO)NHNsample_1not availablesample_cond_1
GFT (4,3)D HCCHsample_1not availablesample_cond_1
SIMULTANEOUS HETERONUCLEAR RESOLVED [1H,1H]-NOESYsample_1not availablesample_cond_1

Software:

DYANA v1.5 - structure solution

NMRPipe v2.3 - processing

CYANA v2.1 - structure solution

CNS v1.1 - refinement

XEASY v1.3 - data analysis

AutoStruct v2.0.0 - data analysis

UBNMR v1.0 - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAK88116 BAP21294 BAP52855 GAA21207 GAJ55663
EMBL CAW29491 CCQ87292 CCQ87507 CDH72943 CDH79240
GB AAG07747 AAT50626 ABJ13629 ABR85041 AEO76930
REF NP_253049 WP_003093986 WP_003103819 WP_003157276 WP_015503515

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts