BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7184

Title: NMR solution structure of CSPsg4   PubMed: 16939212

Deposition date: 2006-06-21 Original release date: 2006-10-17

Authors: Tomaselli, S.; Crescenzi, O.; Sanfelice, D.; Ab, E.; Tancredi, T.; Picone, D.

Citation: Tomaselli, S.; Crescenzi, O.; Sanfelice, D.; Ab, E.; Wechselberger, R.; Angeli, S.; Scaloni, A.; Boelens, R.; Tancredi, T.; Pelosi, P.; Picone, D.. "Solution structure of a chemosensory protein from the desert locust Schistocerca gregaria"  Biochemistry 45, 10606-10613 (2006).

Assembly members:
chemosensory protein CSP-sg4, polymer, 109 residues, Formula weight is not available

Natural source:   Common Name: desert locust   Taxonomy ID: 7010   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Schistocerca gregaria

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
chemosensory protein CSP-sg4: EEKYTTKYDNVNLDEILAND RLLNKYVQCLLEDDESNCTA DGKELKSVIPDALSNECAKC NEKQKEGTKKVLKHLINHKP DVWAQLKAKYDPDGTYSKKY EDREKELHQ

Data sets:
Data typeCount
13C chemical shifts438
15N chemical shifts118
1H chemical shifts700

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1chemosensory protein CSP-sg41

Entities:

Entity 1, chemosensory protein CSP-sg4 109 residues - Formula weight is not available

1   GLUGLULYSTYRTHRTHRLYSTYRASPASN
2   VALASNLEUASPGLUILELEUALAASNASP
3   ARGLEULEUASNLYSTYRVALGLNCYSLEU
4   LEUGLUASPASPGLUSERASNCYSTHRALA
5   ASPGLYLYSGLULEULYSSERVALILEPRO
6   ASPALALEUSERASNGLUCYSALALYSCYS
7   ASNGLULYSGLNLYSGLUGLYTHRLYSLYS
8   VALLEULYSHISLEUILEASNHISLYSPRO
9   ASPVALTRPALAGLNLEULYSALALYSTYR
10   ASPPROASPGLYTHRTYRSERLYSLYSTYR
11   GLUASPARGGLULYSGLULEUHISGLN

Samples:

sample_1: chemosensory protein CSP-sg4, [U-13C; U-15N], 1.2 mM; phosphate buffer 50 mM; D2O 5%; H2O 95%

sample_cond_1: ionic strength: 50 mM; pH: 6.8; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablesample_cond_1
3D 15N-separated NOESYsample_1not availablesample_cond_1

Software:

xwinnmr v3.5 - collection

NMRPipe vlinux - processing

NMRView v5.0.4 - data analysis

CYANA v2.0 - structure solution

AMBER v99 - refinement

NMR spectrometers:

  • Bruker DRX 750 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts