BMRB Entry 7244
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7244
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Title: 1H, 15N, 13C', 13CA and 13CB Chemical Shift Assignments of intrinsically disordered gamma-synuclein PubMed: 17088319
Deposition date: 2006-07-25 Original release date: 2006-11-06
Authors: Marsh, Joseph; Singh, Vinay; Zongchao, Jia; Forman-Kay, Julie
Citation: Marsh, Joseph; Singh, Vinay; Zongchao, Jia; Forman-Kay, Julie. "Sensitivity of secondary structure propensities to sequence differences between a- and g-synuclein: Implications for fibrillation" Protein Sci. 15, 2795-2804 (2006).
Assembly members:
gamma-synuclein, polymer, 127 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
gamma-synuclein: MDVFKKGFSIAKEGVVGAVE
KTKQGVTEAAEKTKEGVMYV
GAKTKENVVQSVTSVAEKTK
EQANAVSEAVVSSVNTVATK
TVEEAENIAVTSGVVRKEDL
RPSAPQQEGEASKEKEEVAE
EAQSGGD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 364 |
| 15N chemical shifts | 125 |
| 1H chemical shifts | 125 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | gamma-synuclein monomer | 1 |
Entities:
Entity 1, gamma-synuclein monomer 127 residues - Formula weight is not available
| 1 | MET | ASP | VAL | PHE | LYS | LYS | GLY | PHE | SER | ILE | ||||
| 2 | ALA | LYS | GLU | GLY | VAL | VAL | GLY | ALA | VAL | GLU | ||||
| 3 | LYS | THR | LYS | GLN | GLY | VAL | THR | GLU | ALA | ALA | ||||
| 4 | GLU | LYS | THR | LYS | GLU | GLY | VAL | MET | TYR | VAL | ||||
| 5 | GLY | ALA | LYS | THR | LYS | GLU | ASN | VAL | VAL | GLN | ||||
| 6 | SER | VAL | THR | SER | VAL | ALA | GLU | LYS | THR | LYS | ||||
| 7 | GLU | GLN | ALA | ASN | ALA | VAL | SER | GLU | ALA | VAL | ||||
| 8 | VAL | SER | SER | VAL | ASN | THR | VAL | ALA | THR | LYS | ||||
| 9 | THR | VAL | GLU | GLU | ALA | GLU | ASN | ILE | ALA | VAL | ||||
| 10 | THR | SER | GLY | VAL | VAL | ARG | LYS | GLU | ASP | LEU | ||||
| 11 | ARG | PRO | SER | ALA | PRO | GLN | GLN | GLU | GLY | GLU | ||||
| 12 | ALA | SER | LYS | GLU | LYS | GLU | GLU | VAL | ALA | GLU | ||||
| 13 | GLU | ALA | GLN | SER | GLY | GLY | ASP |
Samples:
sample_1: gamma-synuclein, [U-13C; U-15N], 0.35 mM; NaCl 137 mM; KCl 2.7 mM; Na2HPO4 10 mM; K2HPO4 2.4 mM; D20 10%
conditions_1: pH: 7.4; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCACB | sample_1 | not available | conditions_1 |
| HNCO | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
| DBJ | BAD97087 BAG35077 BAG73792 |
| EMBL | CAG46587 CAG46589 |
| GB | AAB64109 AAC27738 AAC36550 AAC36586 AAH14098 |
| REF | NP_003078 XP_002820773 XP_003828245 XP_003951754 XP_004049763 |
| SP | O76070 |
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