BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 7271

Title: Backbone and sidechain 1H, 13C and 15N resonance assignments of AF2241 from Archaeoglobus fulgidus   PubMed: 17206469

Deposition date: 2006-08-22 Original release date: 2007-03-29

Authors: Ai, Xuanjun; Yee, Adelinda; Arrowsmith, Cheryl; Li, Shawn; Choy, Wing-Yiu

Citation: Ai, Xuanjun; Semesi, Anthony; Yee, Adelinda; Arrowsmith, Cheryl; Li, Shawn; Choy, Wing-Yiu. "Backbone and side chain 1H, 13C, and 15N resonance assignments of AF2241 from Archaeoglobus fulgidus"  J. Biomol. NMR 38, 183-183 (2007).

Assembly members:
Structural Proteomics protein AF2241, polymer, 153 residues, Formula weight is not available

Natural source:   Common Name: Archaea   Taxonomy ID: 2234   Superkingdom: Archaea   Kingdom: Euryarchaeota   Genus/species: Archaeoglobaceae Archaeoglobus fulgidus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Structural Proteomics protein AF2241: MGTSHHHHHHSSGRENLYFQ GHMGERGVEMRLRIRFESAE CEVELYEEWAPETVRAIADA LPIKSTANRWGDEIYFTTQV AVEKEENSKDVVELGDVAYW IPGKAICLFFGKTPISDDKI RPASAVNVIGRIVNSMEGLK GVADGESVVVERA

Data sets:
Data typeCount
13C chemical shifts501
15N chemical shifts122
1H chemical shifts829

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AF22411

Entities:

Entity 1, AF2241 153 residues - Formula weight is not available

1   METGLYTHRSERHISHISHISHISHISHIS
2   SERSERGLYARGGLUASNLEUTYRPHEGLN
3   GLYHISMETGLYGLUARGGLYVALGLUMET
4   ARGLEUARGILEARGPHEGLUSERALAGLU
5   CYSGLUVALGLULEUTYRGLUGLUTRPALA
6   PROGLUTHRVALARGALAILEALAASPALA
7   LEUPROILELYSSERTHRALAASNARGTRP
8   GLYASPGLUILETYRPHETHRTHRGLNVAL
9   ALAVALGLULYSGLUGLUASNSERLYSASP
10   VALVALGLULEUGLYASPVALALATYRTRP
11   ILEPROGLYLYSALAILECYSLEUPHEPHE
12   GLYLYSTHRPROILESERASPASPLYSILE
13   ARGPROALASERALAVALASNVALILEGLY
14   ARGILEVALASNSERMETGLUGLYLEULYS
15   GLYVALALAASPGLYGLUSERVALVALVAL
16   GLUARGALA

Samples:

sample_1: Structural Proteomics protein AF2241, [U-13C; U-15N], 0.3 mM; NaCl 450 mM; MOPS 10 mM; Zn2+ 10 uM; DTT 10 mM; NaN3 0.01%; Inhibitor mixture 1 x; benzamidine 1 mM

conditions_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_1not availableconditions_1
1H13CHSQCsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
C(CO)NHsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1
HC(CO)NHsample_1not availableconditions_1
15N NOESY-HSQCsample_1not availableconditions_1
13C NOESY-HSQCsample_1not availableconditions_1
(HB)CB(CGCD)HDsample_1not availableconditions_1
(HB)CB(CGCDCE)HEsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian UnityINOVA 600 MHz

Related Database Links:

PDB
GB AAB89017 AIG99248
REF NP_071066 WP_010879730

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts