BMRB Entry 7360
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7360
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Title: 1H, 15N and 13C chemical shift assignments for reduced and oxidised forms of the DsbA oxidoreductase from Vibrio cholerae PubMed: 19636831
Deposition date: 2007-01-10 Original release date: 2007-06-26
Authors: Horne, Henry; Scanlon, Martin
Citation: Horne, James; Scanlon, Martin. "Backbone and side chain 1H, 15N and 13C assignments for the reduced form of the oxidoreductase protein DsbA from Vibrio cholerae" Biomol. NMR Assignments 1, 75-76 (2007).
Assembly members:
DsbA protein disulfide isomerase, polymer, 181 residues, 20443.3 Da.
Natural source: Common Name: cholera Taxonomy ID: 666 Superkingdom: Eukaryota Kingdom: Not applicable Genus/species: Vibrio cholerae
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
DsbA protein disulfide isomerase: AQFKEGEHYQVLKTPASSSP
VVSEFFSFYCPHCNTFEPII
AQLKQQLPEGAKFQKNHVSF
MGGNMGQAMSKAYATMIALE
VEDKMVPVMFNRIHTLRKPP
KDEQELRQIFLDEGIDAAKF
DAAYNGFAVDSMVRRFDKQF
QDSGLTGVPAVVVNNRYLVQ
GQSAKSLDEYFDLVNYLLTL
K
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 533 |
| 15N chemical shifts | 176 |
| 1H chemical shifts | 1156 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DsbA | 1 |
Entities:
Entity 1, DsbA 181 residues - 20443.3 Da.
V148A cloning artefact in original clone gifted to this laboratory
| 1 | ALA | GLN | PHE | LYS | GLU | GLY | GLU | HIS | TYR | GLN | ||||
| 2 | VAL | LEU | LYS | THR | PRO | ALA | SER | SER | SER | PRO | ||||
| 3 | VAL | VAL | SER | GLU | PHE | PHE | SER | PHE | TYR | CYS | ||||
| 4 | PRO | HIS | CYS | ASN | THR | PHE | GLU | PRO | ILE | ILE | ||||
| 5 | ALA | GLN | LEU | LYS | GLN | GLN | LEU | PRO | GLU | GLY | ||||
| 6 | ALA | LYS | PHE | GLN | LYS | ASN | HIS | VAL | SER | PHE | ||||
| 7 | MET | GLY | GLY | ASN | MET | GLY | GLN | ALA | MET | SER | ||||
| 8 | LYS | ALA | TYR | ALA | THR | MET | ILE | ALA | LEU | GLU | ||||
| 9 | VAL | GLU | ASP | LYS | MET | VAL | PRO | VAL | MET | PHE | ||||
| 10 | ASN | ARG | ILE | HIS | THR | LEU | ARG | LYS | PRO | PRO | ||||
| 11 | LYS | ASP | GLU | GLN | GLU | LEU | ARG | GLN | ILE | PHE | ||||
| 12 | LEU | ASP | GLU | GLY | ILE | ASP | ALA | ALA | LYS | PHE | ||||
| 13 | ASP | ALA | ALA | TYR | ASN | GLY | PHE | ALA | VAL | ASP | ||||
| 14 | SER | MET | VAL | ARG | ARG | PHE | ASP | LYS | GLN | PHE | ||||
| 15 | GLN | ASP | SER | GLY | LEU | THR | GLY | VAL | PRO | ALA | ||||
| 16 | VAL | VAL | VAL | ASN | ASN | ARG | TYR | LEU | VAL | GLN | ||||
| 17 | GLY | GLN | SER | ALA | LYS | SER | LEU | ASP | GLU | TYR | ||||
| 18 | PHE | ASP | LEU | VAL | ASN | TYR | LEU | LEU | THR | LEU | ||||
| 19 | LYS |
Samples:
sample-DsbA-reduced: DsbA - Bacterial oxidoreductase, [U-95% 13C; U-90% 15N], 0.3; HEPES 10 mM; NaCl 50 mM
conditions_1: pH: 6.8; temperature: 300 K
conditions_2: pH: 6.8; temperature: 320 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 13C_1H-HSQC | sample-DsbA-reduced | not available | not available |
| 15N_1H-HSQC | sample-DsbA-reduced | not available | not available |
| CBCACONNH | sample-DsbA-reduced | not available | not available |
| HNCACB | sample-DsbA-reduced | not available | not available |
| HNCA | sample-DsbA-reduced | not available | not available |
| HNCO | sample-DsbA-reduced | not available | not available |
| HNCACO | sample-DsbA-reduced | not available | not available |
| CCONHTOCSY | sample-DsbA-reduced | not available | not available |
| HCCONHTOCSY | sample-DsbA-reduced | not available | not available |
| HCCH-TOCSY | sample-DsbA-reduced | not available | not available |
| 13C-NOESY-HSQC | sample-DsbA-reduced | not available | not available |
Software:
SPARKY, UCSF - data visualisation
NMRPipe, NIH - data processing
NMR spectrometers:
- VARIAN AVANCE 600 MHz
- BRUKER DRX 500 MHz
Related Database Links:
| BMRB | 7359 |
| PDB | |
| DBJ | BAP04237 |
| EMBL | CAA45977 |
| GB | AAA27568 AAF93212 ABQ20372 ACP04371 ACP08173 |
| REF | NP_229693 WP_000732279 WP_000732281 WP_000732282 WP_000732283 |
| SP | P32557 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts