BMRB Entry 7364
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR7364
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Title: Solution Structure of Variable-type Domain of Human Receptor for Advanced Glycation Endproducts PubMed: 19032093
Deposition date: 2007-01-19 Original release date: 2009-02-04
Authors: Matsumoto, S.; Yoshida, T.; Yasumatsu, I.; Yamamoto, H.; Kobayashi, Y.; Ohkubo, T.
Citation: Matsumoto, Shigeyuki; Yoshida, Takuya; Murata, Hiroko; Harada, Shusaku; Fujita, Naoko; Nakamura, Shota; Yamamoto, Yasuhiko; Watanabe, Takuo; Yonekura, Hideto; Yamamoto, Hiroshi; Ohkubo, Tadayasu; Kobayashi, Yuji. "Solution Structure of the Variable-Type Domain of the Receptor for Advanced Glycation End Products: New Insight into AGE-RAGE Interaction" Biochemistry 47, 12299-12311 (2008).
Assembly members:
Advanced_glycosylation_end_product-specific_receptor, polymer, 101 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: not available Kingdom: Eukaryota Genus/species: Metazoa Homo
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Advanced_glycosylation_end_product-specific_receptor: AMAQNITARIGEPLVLKCKG
APKKPPQRLEWKLNTGRTEA
WKVLSPQGGGPWDSVARVLP
NGSLFLPAVGIQDEGIFRCQ
AMNRNGKETKSNYRVRVYQI
P
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 401 |
| 15N chemical shifts | 82 |
| 1H chemical shifts | 554 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AG_Endproducts | 1 |
Entities:
Entity 1, AG_Endproducts 101 residues - Formula weight is not available
| 1 | ALA | MET | ALA | GLN | ASN | ILE | THR | ALA | ARG | ILE | ||||
| 2 | GLY | GLU | PRO | LEU | VAL | LEU | LYS | CYS | LYS | GLY | ||||
| 3 | ALA | PRO | LYS | LYS | PRO | PRO | GLN | ARG | LEU | GLU | ||||
| 4 | TRP | LYS | LEU | ASN | THR | GLY | ARG | THR | GLU | ALA | ||||
| 5 | TRP | LYS | VAL | LEU | SER | PRO | GLN | GLY | GLY | GLY | ||||
| 6 | PRO | TRP | ASP | SER | VAL | ALA | ARG | VAL | LEU | PRO | ||||
| 7 | ASN | GLY | SER | LEU | PHE | LEU | PRO | ALA | VAL | GLY | ||||
| 8 | ILE | GLN | ASP | GLU | GLY | ILE | PHE | ARG | CYS | GLN | ||||
| 9 | ALA | MET | ASN | ARG | ASN | GLY | LYS | GLU | THR | LYS | ||||
| 10 | SER | ASN | TYR | ARG | VAL | ARG | VAL | TYR | GLN | ILE | ||||
| 11 | PRO |
Samples:
sample_1: AG_Endproducts, [U-13C; U-15N], 0.2 mM; phosphate buffer 20 mM; Na2SO4 100 mM; D2O 10%; H2O 90%
sample_2: AG_Endproducts, [U-13C; U-15N], 0.2 mM; phosphate buffer 20 mM; Na2SO4 100 mM; D2O 100%
sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D_13C-separated_NOESY | not available | isotropic | sample_conditions_1 |
| 3D_15N-separated_NOESY | not available | isotropic | sample_conditions_1 |
| 2D NOESY | not available | isotropic | sample_conditions_1 |
| 2D TOCSY | not available | isotropic | sample_conditions_1 |
Software:
CNS v1.1, Brunger - refinement
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 500 MHz
Related Database Links:
| BMRB | 17378 19739 19953 |
| PDB | |
| DBJ | BAA05958 BAA89369 BAC65465 BAG35995 BAG60385 |
| GB | AAA03574 AAB47491 AAH20669 AAX07272 AAX07273 |
| REF | NP_001127 NP_001192046 NP_001193858 NP_001193863 NP_001193865 |
| SP | Q15109 |
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