BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10033

Title: Soluiotn structure of J-domain of mouse DnaJ like protein

Deposition date: 2006-11-01 Original release date: 2008-08-13

Authors: Kobayashi, N.; Koshiba, S.; Inoue, M.; Tochio, N.; Tomizawa, T.; Kigawa, T.; Yokoyama, S.

Citation: Kobayashi, N.; Koshiba, S.; Inoue, M.; Tochio, N.; Tomizawa, T.; Kigawa, T.; Yokoyama, S.. "Solution Structure of J-domain of mouse DnaJ like protein"  . ., .-..

Assembly members:
J-domain, polymer, 94 residues, Formula weight is not available

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
J-domain: GSSGSSGMALEQTLKKDWYS ILGADPSANMSDLKQKYQKL ILLYHPDKQSADVPAGTMEE CMQKFIEIDQAWKILGNEET KKKYDLQRSGPSSG

Data sets:
Data typeCount
13C chemical shifts401
15N chemical shifts92
1H chemical shifts638

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
11700030A21Rik protein1

Entities:

Entity 1, 1700030A21Rik protein 94 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYMETALALEU
2   GLUGLNTHRLEULYSLYSASPTRPTYRSER
3   ILELEUGLYALAASPPROSERALAASNMET
4   SERASPLEULYSGLNLYSTYRGLNLYSLEU
5   ILELEULEUTYRHISPROASPLYSGLNSER
6   ALAASPVALPROALAGLYTHRMETGLUGLU
7   CYSMETGLNLYSPHEILEGLUILEASPGLN
8   ALATRPLYSILELEUGLYASNGLUGLUTHR
9   LYSLYSLYSTYRASPLEUGLNARGSERGLY
10   PROSERSERGLY

Samples:

sample_1: J-domain of DnaJ like protein, [U-13C; U-15N], 1.07 mM; Phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.901, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAB24631
GB AAH30072 AAH91774 AAK21968 EDL27758
REF NP_081268
SP Q91ZF0

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts