BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 10092

Title: Solution structure of the DEATH domain of Interleukin-1 receptor-associated kinase4 (IRAK4) from Mus musculus

Deposition date: 2007-01-24 Original release date: 2008-08-14

Authors: Nameki, N.; Tomizawa, T.; Koshiba, S.; Kigawa, T.; Yokoyama, S.

Citation: Nameki, N.; Tomizawa, T.; Koshiba, S.; Kigawa, T.; Yokoyama, S.. "Solution structure of the DEATH domain of Interleukin-1 receptor-associated kinase4 (IRAK4) from Mus musculus"  . ., .-..

Assembly members:
DEATH domain, polymer, 127 residues, Formula weight is not available

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
DEATH domain: GSSGSSGMNKPLTPSTYIRN LNVGILRKLSDFIDPQEGWK KLAVAIKKPSGDDRYNQFHI RRFEALLQTGKSPTCELLFD WGTTNCTVGDLVDLLVQIEL FAPATLLLPDAVPQTVKSLP PSGPSSG

Data sets:
Data typeCount
13C chemical shifts531
15N chemical shifts112
1H chemical shifts857

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1interleukin-1 receptor-associated kinase 41

Entities:

Entity 1, interleukin-1 receptor-associated kinase 4 127 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYMETASNLYS
2   PROLEUTHRPROSERTHRTYRILEARGASN
3   LEUASNVALGLYILELEUARGLYSLEUSER
4   ASPPHEILEASPPROGLNGLUGLYTRPLYS
5   LYSLEUALAVALALAILELYSLYSPROSER
6   GLYASPASPARGTYRASNGLNPHEHISILE
7   ARGARGPHEGLUALALEULEUGLNTHRGLY
8   LYSSERPROTHRCYSGLULEULEUPHEASP
9   TRPGLYTHRTHRASNCYSTHRVALGLYASP
10   LEUVALASPLEULEUVALGLNILEGLULEU
11   PHEALAPROALATHRLEULEULEUPROASP
12   ALAVALPROGLNTHRVALLYSSERLEUPRO
13   PROSERGLYPROSERSERGLY

Samples:

sample_1: DEATH domain, [U-13C; U-15N], 0.7 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.5; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1not availablecondition_1
3D 13C-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.901, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - structure solution

OPALp, Billeter, M., Guntert, P., Koradi, R. - refinement

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAB32090 BAC26146 BAE24205
GB AAH51676 AAM15773 ABK42447 EDL04264 EDL04265
REF NP_084202 XP_011243947
SP Q8R4K2

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts